BDNF对BMSCs向脑出血灶周围组织迁移的保护作用  

Protective effect of brain derived neurotrophic factor on mesenchymal stem cells when transplanting to the surrounding issue of intracerebral hemorrhage stove

作　　者：任瑞芳[1] 黄良国[1] 黄名璐[1] 蒋国红[1] 白洁[1] 

机构地区：[1]遵义医学院附属医院神经内科,遵义563000

出　　处：《中华神经医学杂志》2014年第3期252-256,共5页Chinese Journal of Neuromedicine

基　　金：贵州省科学技术厅社会发展攻关资助项目（黔科合SY20103086）

摘　　要：目的观察脑源性神经营养因子（BDNF）基因重组慢病毒转染骨髓间质干细胞（BMSCs）后，被移植人大鼠侧脑室内，向对侧脑出血灶周围组织的迁移情况。方法将60只脑出血模型大鼠按随机数字表法分为磷酸盐缓冲液（PBS）组、骨髓间质干细胞（BMSCs）gg、骨髓间质干细胞一绿色荧光蛋白组（BMSCs-EGFP）、脑源性神经营养因子（BMSCs．EGFP．BDNF）组，每组15只。分别向侧脑室注射PBS，BMSCs，慢病毒（LV）转染的EGFP，LV转染的BDNF．EGFP，在术后7d、14d、21d采用Westenblotting检测各组BDNF在BMSCs中的蛋白表达．免疫荧光检测Brdu标记的BMSCs，EGFP及BDNF。结果Westernblotting检测BMSCs．EGFP—BDNF组BDNF蛋白表达明显多于BMSCs组及BMSCs．EGFP组畔0．05）；细胞爬片BMSCs．EGFP．BDNF组BDNF荧光表达明显高于BMSCs组及BMSCs．EGFP组：脑组织切片免疫荧光单标显示BMSCs．EGFP．BDNF组迁移至脑出血灶周围组织的BMSCs阳性细胞数明显多于BMSCs组及BMSCs．EGFP组fP〈0．05）．BMSCs组与BMSCs-EGFP组除7d外，其余比较差异无统计学意义f胗0．05）。结论基因重组慢病毒修饰的BMSCs中BDNF表达增高，提示BDNF对BMSCs向脑出血灶周围组织迁移具有保护作用。Objective To observe the migration of mesenchymal stem cells （MSCs） transfected with recombinant lentiviral vectors carried brain derived neurotrophic factor （BDNF） gene from lateral ventricle to intracerebral hemorrhage stove in rats and to discuss the protective effect of BDNF on MSCs. Methods Intracerebral hemorrhagic models were constructed in 60 SD rats and randomly divided into 4 groups： phosphate buffer solution （PBS） group, BMSCs group, BMSCs-enhanced green fluorescent protein （EGFP） group and BMSCs-EGFP-BDNF group （n=15）; PBS, BMSCs, lentiviral vector （LV） carried EGFP and LV carried BDNF-EGFP were, respectively, injected into the lateral cerebral ventricle of each group; 7, 14 and 21 d after the injection, BDNF protein expression in the BMSCs of each group was detected by Western blotting and immunofluorescence; the migration of BMSCs ~om lateral ventricle to intracerebral hemorrhage stove was observed by signal labeling immunofluorescent staining. Results Western blotting and immunofluorescence showed that the BDNF protein expression in the MSCs-EGFP-BDNF group was significantly higher than that in the MSCs group and MSCs-EGFP group （P〈0.05）; signal labeling immunofluorescent staining of brain tissue section indicated that the MSCs-EGFP-BDNF group had significantly larger number of BMSCs-positive cells which migrated to the surrounding issues of intracerebral hemorrhage stove than MSCs group and MSCs-EGFP group （P〈0.05）, while there were no significant differences between the latter two except on the 7th d of establishment of models. Conclusion BMSCs modified with recombinant LV carried BDNF gene have high expression level of BDNF, indicating that BDNF performs a protective effect on BMSCs.

关 键 词：间质干细胞 慢病毒属 脑源性神经营养因子 细胞迁移分析 

分 类 号：R743.34[医药卫生—神经病学与精神病学]