氦气预适应对大鼠局灶性脑缺血再灌注损伤的保护作用  

Protective effect of helium preconditioning on focal cerebral ischemia-reperfusion injury in rats

作　　者：周春辉[1,2] 张剑宁[1] 程岗[1] 张荣佳 

机构地区：[1]海军总医院神经外科,北京100048 [2]第二军医大学研究生院,上海200433 [3]海军医学系潜水教研室

出　　处：《中华神经医学杂志》2014年第3期257-260,共4页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金面上项目（81372128）

摘　　要：目的观察氦气预适应对急性局灶性脑缺血再灌注损伤大鼠的保护作用，并初步探讨其可能机制。方法36只SD大鼠按随机数学表法分为3组：假手术组、模型组和氦气预适应组（每组各12只），后2组采用线栓法复制成大鼠大脑中动脉缺血2h再灌注24h损伤模型，氦气预适应组于大脑中动脉闭塞（MCAO）模型制备前24h行氦气f氦气：氧气=80％：20％、暴露5minx3次、每次间隔空气5min。再灌注24h后对各组大鼠进行神经功能缺损评分和脑缺血再灌注损伤程度（脑缺血面积百分比、缺血区质量百分比1检测，以及采用BCA蛋白定量法测定超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量，同时显微镜下观察脑组织尼氏染色情况。结果与模型组比较，氦气预适应组大鼠神经功能缺损评分和脑缺血再灌注损伤程度显著缓解，脑组织SOD活性明显增高、MDA含量明显降低，差异均有统计学意义fP〈0．05）。同时氦气预适应组大鼠较模型组脑组织皮层及海马的存活神经元数量明显增加。结论氦气预适应对大鼠急性局灶性脑缺血再灌注损伤有一定的保护作用，其机制可能与清除氧自由基有关。Objective To study the protective effect of helium preconditioning （HPC） on rats with acute transient focal cerebral ischemia/reperfusion （I/R） injury and its possible mechanism. Methods Healthy adult SD rats were divided into 3 groups： sham-operated group, I/R group and HPC group; the rat models of focal cerebral I/R in the later two groups were established by middle cerebral artery occlusion （MCAO, 2 h） and reperfusion （24 h）. Rats in the HPC group were exposed to the helium for 5 minx3 times （5 rain interval） 24 h before MCAO. Neurological deficit scale was performed; the degrees of cerebral injury （infarct volume） were evaluated; BCA protein quantification was used to measure the superoxide dismutase （SOD） activity and malondialdehyde （MDA） content. The morphological changes of the brain were observed by Nissl staining. Results As compared with those in the I/R group, the neurological deficit scale scores were deceased, inl^acted volume and content of MDA were decreased, and SOD activity was increased in HPC group, with significant differences （P〈 0.05）; the number of surviving neurons in the brain cortex and hippocampus of HPC group was increased as compared with that in the FR group. Conclusion HPC has a protective effect against acute focal cerebral I/R injury, whose mechanism may be related to scavenging of fxee radicals by spermine.

关 键 词：氦气 预适应 脑缺血再灌注 超氧化物歧化酶 丙二醛 

分 类 号：R743[医药卫生—神经病学与精神病学]