IL-1β诱导子宫内膜异位症间质细胞activin A及相关因子的表达  

Effect of interleukin-1β on expressions of activin A and its related factors in cultured endometrial stromal cells from patients with endometriosis

作　　者：李蕾[1] 刘木彪[1] 朱峰城 潘颖[1] 

机构地区：[1]南方医科大学珠江医院妇产科,广东广州510282

出　　处：《南方医科大学学报》2014年第3期410-413,共4页Journal of Southern Medical University

基　　金：广东省科技计划项目(2010B031600096);广东省中医药局科研项目(2010228);广东省医学科研基金(C2010008);广州市民生科技重大专项(11BppZXaa2120016)

摘　　要：目的探讨白细胞介素1β(IL-1β)对子宫内膜异位症(内异症)在位内膜间质细胞activin A、follistatin、cripto的表达及蛋白分泌的影响。方法分离培养内异症在位内膜间质细胞,加入不同浓度IL-1β(250 pg/ml,500 pg/ml,750 pg/ml)干预。实时荧光定量PCR技术及酶联免疫吸附试验,分别从mRNA及蛋白水平检测activin A、follistatin、cripto的变化。结果经IL-1β刺激,activin A及follistatin mRNA表达及蛋白分泌较对照组呈IL-1β剂量依赖性增加;cripto mRNA表达较对照组呈IL-1β剂量依赖性增加。结论 IL-1β可影响内异症在位内膜activinA、follistatin、cripto的分泌及表达。Objective To study the effect of interleukin-1β（IL-1β） on the expressions activin A, follistatin, and cripto in cultured human endometrial stromal cells （HESCs） form patients with endometriosis. Methods Cultured HESCs were stimulated with 250, 500, and 750 pg/ml IL-1β, and the mRNA and protein expressions of activin A, follistatin, and cripto were assayed using real-time reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay. Results IL-1βtreatment caused significant dose-dependent increments of the mRNA and protein expressions of activin A and follistatin and of the mRNA expression of cripto in cultured HESCs. Conclusion IL-1βcan affect the expressions of activin A, follistatin and cripto in HESCs from patients with endometriosis.

关 键 词：子宫内膜异位症 白细胞介素-1Β INTERLEUKIN-1Β 

分 类 号：R711.71[医药卫生—妇产科学]