BMP9对人肺腺癌A549细胞侵袭、迁移的影响及机制的研究  

Effects and Possible Mechanisms of BMP9 on the Invasion and Migration of Human Lung Cancer A549 Cells

作　　者：王建华[1] 唐治贵[1] 袁永强[1] 代红莹[1] 王科[1] 

机构地区：[1]重庆医科大学附属永川医院检验科,重庆402160

出　　处：《中国细胞生物学学报》2014年第2期177-183,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:30800658);重庆医科大学附属永川医院院内课题(批准号:YJYB201313)资助的课题~~

摘　　要：BMP9属于TGF-β超家族的成员,参与多种细胞的增殖、分化、凋亡、侵袭、转移过程。以人肺腺癌细胞A549作为目的细胞,采用腺病毒体外感染方式,外源性高表达BMP9。RTPCR及Western blot检测重组细胞中BMP9的表达,通过细胞划痕实验、Transwell侵袭实验检测AdBMP9细胞侵袭及迁移改变,RT-PCR及Western blot检测感染BMP9腺病毒后IL-6的mRNA和蛋白表达;Western blot检测PI3K/Akt信号通路中总Akt和磷酸化Akt蛋白的表达。结果显示:与对照组细胞相比,感染BMP9腺病毒后,A549中BMP9 mRNA和蛋白表达明显升高;实验组划痕愈合率由对照的(85.4±2.1)%与(86.5±3.4)%上升至(97.4±2.6)%(P<0.05);实验组穿膜细胞数由对照的(115.5±13.1)个与(123.3±14.9)个上升至(224.3±24.6)个(P<0.05);与对照组细胞相比,AdBMP9组IL-6的表达上调,磷酸化Akt蛋白表达上调。该研究表明,BMP9可能通过上调IL-6的表达,激活PI3K/Akt信号通路,促进人肺腺癌A549的侵袭、迁移。Bone morphogenetic protein 9 （BMP9）, which belongs to the transforming growth factor-β superfamily, regulates a wide range of cellular responses including cell proliferation, differentiation, adhesion, migration, and apoptosis. Human lung cancer A549 cells were used as experiment cells and the over-expression of BMP9 was infected by adenovirus in vitro. RT-PCR and Western blot were used to detect the expression of BMP9 in recombi- nant AdBMP9 cells; To detect the changes of the recombinant AdBMP9 cells＇ invasion and migration, cell wounding assay and Transwell invasion assay were carried out. At last, RT-PCR and Western blot were used to detect the expression of IL-6, and Western blot was used to detect the expression of total Akt and phosphorylation Akt after BMP9 adenovirus infection. These results showed that BMP9 mRNA and protein were expressed in recombinant AdBMP9 cells; The healing rate of experimental group was increased from （85.4±2.1）% and （86.5±3.4）% in control groups to （97.4±2.6）% （P〈0.05）, and the number of invasion cells which moved across the matrix barrier in experimental group was increased from （105.5±13.1） and （113.3±14.9） in control groups to （224.3±24.6） （P〈0.05）. In compari- son with the control groups, the expression of IL-6 and phosphorylated Akt were significantly increased in Ad- BMP9 group. We can draw the conclusion that BMP9 can promote the invasion and migration of lung cancer A549 cells possibly through up-regulation of IL-6 and activation of the PI3k/Akt cell signaling pathway.

关 键 词：骨形态发生9 肺癌 白细胞介6 P13K AKT信号通路 

分 类 号：R734.2[医药卫生—肿瘤]