4-磺酸酯-2,2,6,6-四甲基哌啶氮氧自由基对大鼠组织和红细胞的抗脂质过氧化作用  

Antioxidative activity of 4-sulfonate-2,2,6,6-tetramethylpiperidi- neoxyl in tissues and erythrocytes from rats

作　　者：葛斌[1] 陈立德[1] 张振明[1] 

机构地区：[1]甘肃省人民医院药剂科,甘肃兰州730000

出　　处：《中国药理学与毒理学杂志》2001年第1期47-50,共4页Chinese Journal of Pharmacology and Toxicology

摘　　要：研究了 4 磺酸酯 2 ,2 ,6,6 四甲基哌啶氮氧自由基 (STMP)的抗脂质过氧化作用 .结果表明 ,STMP显著抑制丙二醛 (MDA)的生成 ,其抑制心 ,肝 ,肾MDA生成的IC50 分别为 4 .4 ,3.2及 3.7μmol·L- 1;对抗溶血反应 ,其IC50 为 318.3μmol·L- 1;但不影响O2 ÷ 的生成 .The effect of antioxidation of 4 sulfonate 2,2,6,6 tetramethylpiperidineoxyl (STMP) in tissues and erythrocytes from rats were studied by determining malondialdehyde (MDA) formation induced by ·OH free radical generation system (Fe 2+ ascorbic acid) in homogenate of heart, liver and kidneys of rats using thiobarbituric acid colorimetric method, by measuring spectrometrically H 2O 2 caused hemolysis and by assaying superoxide anion from zymosan stimulated neutrophils of rats. The results showed that STMP markedly inhibited MDA generation caused by ·OH generation system with IC 50 value 4.4(in heart), 3.2(in liver) and 3.7(in kidneys) μmol·L -1 , respectively, antagonized H 2O 2 caused hemolysis (IC 50 =318 μmol·L -1 ), but did not affect O 2 ÷ formation from activated neutrophils. It is suggested that STMP exert their antilipoperoxidative effect by specifically scavenging ·OH free radicals and H 2O 2 in biological system.

关 键 词：磺酸酯 STMP 四甲基哌啶氮氧自由基 丙二醛 中性 

分 类 号：R73-3[医药卫生—肿瘤]