贵州苗族、布依族、侗族人群线粒体DNA多态性研究  

Genetic Polymorphism of Mitochondrial DNA in Ethnic Miao,Buyi and Dong from Guizhou

作　　者：何燕[1] 单可人[1] 任凌雁[1] 张婷[1] 王婵娟[1] 官志忠[1] 

机构地区：[1]贵阳医学院分子生物学重点实验室,贵州贵阳550004

出　　处：《生物技术》2014年第1期61-66,共6页Biotechnology

基　　金：国家"十二五"科技支撑计划项目(编号:2013BAI05B03);贵州省科技厅社会发展攻关项目(编号:SY[2012]3135;SY[2010]3001]);贵州省科技厅基金项目(编号:J[2011]2119)资助

摘　　要：目的:调查贵州苗族、布依族、侗族人群mtDNA的群体遗传特点。方法:用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术和DNA测序法,对3个民族男性个体mtDNA上12个SNP进行多态性分析。结果:285名个体共检出47种单倍型和12种单倍群。主要单倍型H32、H36、H10在3个民族间频率分布差异有统计学意义(P<0.05);3个民族共有单倍型为11种(72.98%)且在民族间频率分布差异有统计学意义(P<0.05),单倍群B(21.05%)和M7(21.05%)频率最高,B在苗族、布依族中分布频率与侗族间差异有统计学意义(P<0.05)。结论:贵州苗族、布依族、侗族人群mtDNA单倍型分布相似,单倍群频率及主成分分析表现出南方群体特征。侗族母系遗传结构较苗族和布依族更为复杂,民族间存在基因交流的可能。Objective：To study the population genetics characteristics of mitochondrial DNA in ethnic Miao, Buyi and Dong populations from Guihzou province. Method：Polymerase chain reaction and restriciton fragment length polymorphism （PCR - RFLP） was used to de- tect the 12 SNP loci in a total of 285 samples. The result was verified with DNA sequencing. Result：The 47 haplotypes and 12 haplogroups were detected in 285 samples. The main haplotypes were H32, H36 and H10 （41.04%）, but the difference was statistically significant a- mong the three ethnic groups （P 〈 0.05）. There were 11 haplotypes were found in all the groups. By the way, H11 was only found in Miao population （11.58% ）. Haplogroup B and M7 were the predominant types （both of 21.05% ）. In haplogroup B, Miao and Buyi had differ- ence with Dong respectively and the difference was statistically significant （P 〈 0. 05 ）. Conclusion： There was greatly similarity in the dis- tribution of the haplotype of the mtDNA among the three ethnic groups, but there were some difference in the distribution of haplo- types. Haplogroups and Principal Component （PC） analysis showed that the genetic polymorphism of mtDNA of the three populations indi- cated their origination from southern China. The matrilineal genetic structure of Dong population is more complex than the others, but they all have the possibility of gene flow.

关 键 词：苗族 侗族 布依族 线粒体DNA 遗传多态性 

分 类 号：Q987[生物学—遗传学]