CD4^+IL-9^+细胞在大肠癌免疫微环境中的分布及临床意义  

Distribution and clinical significance of CD4^+ IL-9^+ lymphocyte in immune microenvironment of colorectal cancer

作　　者：张磊昌[1] 陈利生[1] 张森[1] 龙军先[1] 黄永红[1] 钟武[1] 高枫[1] 曹云飞[1] 

机构地区：[1]广西医科大学第一附属医院结直肠肛门外科,广西南宁530021

出　　处：《中华肿瘤防治杂志》2014年第6期407-410,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(81260316)

摘　　要：目的:检测CD4+IL-9+(Th9)细胞在大肠癌免疫微环境中的表型和分布特征,分析其与临床病理的相关性。方法:选取2013-06-05-2013-10-10广西医科大学第一附属医院初次行手术治疗的45例大肠癌患者的肿瘤组织、相对应癌旁组织以及外周血(术前和术后),同时抽取15例健康志愿者外周血,用流式细胞仪检测各标本中Th9、Th1和Th2在CD4+细胞中的比例,记录患者的临床病理资料(性别、年龄、民族、Ducks分期、肿瘤大小、淋巴结转移、CEA和AFP水平);另选取5例同期肠癌患者的肿瘤组织对Th9细胞表型特征(CD45RO/CD45RA)进行流式细胞仪测定。结果:肠癌组织中的Th9细胞比例为(1.12±0.33)%,明显高于癌旁组织(0.85±0.29)%和术前外周血(0.65±0.27)%,P值均<0.05;肠癌组织中Th9细胞表面高表达CD45RO蛋白为(85.23±5.91)%,低表达CD45RA蛋白为(18.23±6.78)%;术前外周血Th9细胞比例为(0.65±0.27)%,明显高于术后外周血(0.47±0.24)%,P=0.001 2;Th9细胞与肠癌临床病理相关性分析表明,其与Ducks分期有关,P<0.05,与其他因素无关;肠癌组织中Th9细胞比例与Th1细胞无明显相关,r=0.140,P=0.360,与Th2细胞比例呈负相关,r=-0.65,P<0.001。结论:Th9淋巴细胞在大肠肿瘤微环境存在聚集现象,其与肿瘤的进展有关,可能参与了大肠癌免疫微环境的调节进而影响了肿瘤的发生和发展。OBJECTIVE： To detect the distribution of CD4^＋ IL-9^＋ （Th9） lymphocyte in immune microenvironment of colorectal cancer （CRC） and analyze its relation with clinical pathology. METHODS.. Forty-five patients with CRC who under- went surgery for the first time from 2013-06-05 to 2013-10-10 in First Affiliated Hospital of Guangxi Medical University were enrolled,tumor and relevant para-carcinoma tissue, pre or post-operative peripheral blood （PB） of these patients, and PB from 15 healthy volunteers were detected for the proportion of Th9, Tb1, Th2 in CD4^＋ lymphocyte by flow cytometry（FCM）. Clinic pathologic data like sexy, age, nation, Ducks stage, tumor size, metastasis of lymphocyte, lever of CEA and AFP was recorded. Tumor tissue of another 5 patients with CRC underwent FCM for analysis of expression of CD45RO/CD45RA. RESULTS： The proportion of Th9 in tumor tissue was （1. 12±0. 33）% which was significantly higher than that in para-carcinoma tissue （0. 85±0.29）% and PB （0.65 ±0.27）%, respectively （P〈0.05）. Th9 in tumor tissue showed high expression of CD45RO （85.23±5.91 ）% and low expression CD45 RA（ 18.23±6.78） %. The poroportion of Th9 in PB before operation was higher than that after operation （0.47±0.24） % （P=0. 0012）. Th9 lymphocyte was concerned with Ducks stage （P〈0.05） ,while it had nothing to do with other factors, there was no significant correlation between the proportion of Th9 in tumor tissue and Th1 （r= -0. 140,P=0. 360） ,while negative correlation existed between Th9 and Th2 （r= -0.65,P〈0. 001）. CONCLUSION.. There is enrichment phenomenon of Th9 in the immune microenvironment of CRC, Th9 is related to the progression of CRC and may take part in regulating the immune microenvironment of CRC and play an important role in the progress of CRC.

关 键 词：肠肿瘤 CD4^+IL-9^+ 免疫微环境 T辅助淋巴细胞 

分 类 号：R735.34[医药卫生—肿瘤]