非小细胞肺癌组织MYLK和MYL9表达临床意义探讨  

Expressions of MYLK and MYL9 and their clinical significance in non-small cell lung cancer

作　　者：谭翔[1] 陈铭伍[1] 王永勇[1] 冼磊[1] 郭建极[1] 阳诺[1] 郭钦明[1] 

机构地区：[1]广西医科大学第一附属医院心胸外科,广西南宁530021

出　　处：《中华肿瘤防治杂志》2014年第6期433-438,共6页Chinese Journal of Cancer Prevention and Treatment

基　　金：广西科学研究与技术开发计划(桂科攻10124001A-47)

摘　　要：目的:探讨肌球蛋白轻链激酶(myosin light chain kinase,MYLK)和肌球蛋白轻链(myosin regulatory light chain9,MYL9)在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织的表达情况及临床意义。方法:采用qRT-PCR及蛋白质印迹法检测2012-08-20-2013-02-01广西医科大学第一附属医院胸外科30例NSCLC组织和对应的癌旁组织及30例正常肺组织中MYLK和MYL9mRNA及蛋白的表达情况。进一步对影响MYLK和MYL9表达的临床特征进行Logistic回归分析。结果:NSCLC癌组织MYLK基因相对表达量为0.50±0.19,癌旁组织为0.97±0.22,正常组织为1,癌组织低于癌旁(P=0.001)及正常组织(P<0.001);MYLK蛋白在肺癌组织、癌旁组织及正常组织中表达分别为0.54±0.18、0.72±0.21和0.84±0.23,癌组织低于癌旁(P=0.008)及正常组织(P=0.003);MYL9基因在肺癌组织、癌旁组织及正常组织相对表达量分别为0.37±0.12、0.95±0.19和1,癌组织低于癌旁(P<0.001)及正常组织(P<0.001);MYL9蛋白在肺癌组织、癌旁组织及正常组织中蛋白相对表达量分别为0.56±0.14、0.74±0.20和0.78±0.19,癌组织低于癌旁(P=0.001)及正常组织(P<0.001)。MYLK和MYL9的mRNA及蛋白相对表达量与病理类型、分化程度和临床分期无关。癌组织淋巴结转移组MYLK的mRNA(P=0.04)和蛋白(P=0.04)及MYL9的mRNA(P<0.001)和蛋白(P=0.024)相对表达量明显高于无淋巴结转移组;吸烟组MYLK的mRNA(P<0.001)和蛋白(P=0.038)及MYL9的mRNA(P=0.001)及蛋白(P=0.01)相对表达量低于无吸烟组;男性MYLK的mRNA(P<0.001)和蛋白(P=0.03)及MYL9的mRNA(P=0.001)和蛋白(P<0.001)相对表达量低于女性组。多因素分析显示,女性、无吸烟及淋巴结转移增加MYLK及MYL9的表达。结论:NSCLC中MYLK和MYL9mRNA及蛋白表达均明显低于癌旁及正常组织,且在淋巴结转移的癌组织中表达均高于无淋巴结转移组。女性、无吸烟及淋巴结转移是MYLK及MYL9表达量增高的主要影响因素。OBJECTIVE： To explore the clinical significance of MYLK and MYL9 expression in non- small cell lung cancer（NSCLC）. METHODS： Thirty cases of non-small cell lung cancer tissue, corresponding paraneoplastic tissues and 30 cases of normal lung tissues from 2012-08-20 to 2013-02-01 in department of cardiothoracic surgery of First Affiliated Hospital of Guangxi Medical University were detected by quantitative Real-time PCR and Western blot methods. The clin- ical features of expression of MYLK and MYL9 were analyzed by Logistic regression analysis. RESCLTS.. The relative ex- pression of MYLK mRNA and protein in non-small cell lung cancer was 0.50±0.19 and 0. 54±0.18,which was signifi- cantly lower than that in the paraneoplastic tissues （0.97 ± 0.22,0. 72 ± 0.21） and normal tissues （ 1,0. 84 ± 0.23, all P values 〈0.05）,paraneoplastic tissues and normal tissues were found no statistically significant difference（P〉0.05）. While in the MYL9,the relative expression of MYL9 mRNA and protein in non-small cell lung cancer was 0. 37±0.12 and 0.56±0.14 ,which was significantly lower than that in the paraneoplastic tissues （0. 95±0.19,0. 74±0.20） and nor- mal tissues （1,0.78±0.19, all P〈0.05）, paraneoplastic tissues and normal tissues were found no statistically significant difference（all P〉0.05）. MYLK mRNA and MYL9 mRNA in lymph node metastasis and non- metastasis （MYLK：P= 0.04;MYL9：P〈0.001）,smoking and non-smoking（MYLK：P〈0. 001; MYL9： P= 0. 001） and male and female （MYLK：P〈0. 001 ;MYL9：P=0. 001） were statistically significant different,whereas in MYLK and MYL9 protein ex- pression in lymph node metastasis and non- metastasis（ MYLK： P= 0.04 ; MYL9 ： P = 0. 024 ）, smoking and non-smoking （MYLK：P=0. 038;MYL9：P=0.01） and male and female （MYLK：P=0.03;MYL9：P〈0. 001）. The multiple regres- sion analysis showed that only female, non-smoking and lymphatic node metastasis increased MYLK and MYL9 expression markedly. CONCLUSIONS： Com

关 键 词：癌 非小细胞肺 MYLK MYL9 实时荧光定量PCR 蛋白质印迹 基因表达 

分 类 号：R734.2[医药卫生—肿瘤]