前列腺癌组织Vitronectin表达临床意义研究  

Expression of Vitronectin in the blood serum of prostate cancer and its role in prostate cancer with metastasis

作　　者：舒博[1] 张玲[2] 牛越[1] 陈鹏[1] 

机构地区：[1]新疆医科大学附属肿瘤医院泌尿科,新疆乌鲁木齐830011 [2]新疆维吾尔自治区疾病预防控制中心,新疆乌鲁木齐830002

出　　处：《中华肿瘤防治杂志》2014年第6期447-450,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：新疆维吾尔自治区自然科学基金(2010211A29);中国博士后科学基金面上资助第45批项目(20090451516)

摘　　要：目的:观察玻连蛋白(vitronectin,VTN)在前列腺癌组织及患者血清中的表达,并探讨其临床意义及与前列腺癌转移的相关性。方法:应用免疫组织化学法检测2010-01-01-2012-12-31新疆医科大学附属肿瘤医院泌尿外科34例前列腺癌转移(prostate cancer metastasis,PCaM)患者、32例前列腺癌未转移(prostate cancer,PCa)患者及29例良性前列腺增生(benign prostatic hyperplasia,BPH)患者组织中VTN表达,通过χ2检验比较各组组织中VTN的表达差异,分析前列腺癌组织中VTN表达与临床病理因素的相关性;同时用蛋白印迹法检测相应患者血清中VTN表达,通过方差分析及SNK法检验各组血清中VTN的表达差异。结果:BPH组、PCa组及PCaM组组织样本中VTN的阳性表达率分别为82.76%(24/29)、59.38%(19/32)及32.35%(11/34),VTN在BPH组组织中的表达水平高于PCa组,PCa组高于PCaM组,χ2=16.34,P<0.001;前列腺癌组织中VTN表达与患者初诊血清PSA(P=0.004)、肿瘤临床分期(P=0.025)及Gleason评分(P=0.004)存在相关性;VTN在BPH组血清样本中的光密度比值为0.83±0.07,高于PCa组0.65±0.06,PCa组高于PCaM组0.35±0.08,P<0.001。结论:VTN与前列腺癌的发生和转移可能相关,并可能在前列腺癌的进展中起重要作用,借助测定血清VTN的表达可能可以协助前列腺癌的诊断和疾病进展评估。OBJECTIVE： To detect the expression of Vitronectin （VTN） in the tissues and blood serum of prostate cancer,and evaluate its clinical significances and role in prostate cancer with metastasis. METHODS： Immunohistochemis- try and Western blot method were used to detect VTN expression in 34 prostate cancer patients with metastasis （PCaM）, 32 prostate cancer patients without metastasis （PCa） and 29 benign prostatic hyperplasia patients （BPH） from Depart- ment of Urology,Tumor Hospital Affiliated Xinjiang Medical University from 2010-01-01 to 2012-12-31. VTN experssion in different groups and the relation between the expression of VTN protein and clinicopathologic parameters in prostate cancer were analyzed. RESULTS： The positive expression rate of VTN in BPH, PCa and PcaM was 82. 76% （24/29）, 59.38% （19/32） and 32.35% （11/34） ,there was a significant difference in each group （X^2 =16.34,P〈0. 001）. VTN pro- tein expression was significantly associated with serum PSA （P= 0. 004）, tumor stage （P= 0. 025） and Gleason score （P=0. 004） in prostate cancer. VTN was highly expressed in the blood serum of BPH patients（0. 83±0.07） than that of PCa patients（0.66±0.06）. VTN was also highly expressed in the blood serum of PCa patients than that of PCaM patients （0.35±0.08,P〈0. 001）. CONCLUSIONS： VTN may be related to the malignant transformation and metastatis of pros- tate cancer. Detecting the expression of VTN may be helpful in evaluating the diagnosis and prognosis of prostate cancer.

关 键 词：玻连蛋白 前列腺肿瘤 免疫组织化学法 蛋白印迹法 

分 类 号：R737.25[医药卫生—肿瘤]