高效液相色谱法测定保健品中姜黄素含量的研究  

A Study on Determination of Curcumin in Health Food by HPLC

作　　者：单胜艳[1] 张丹[2] 王春茹[1] 刘文涵[3] 郭晓风[1] 

机构地区：[1]杭州娃哈哈集团有限公司研究院,浙江杭州310018 [2]盐城出入境检验检疫局,江苏盐城224002 [3]浙江工业大学化学工程与材料学院,浙江杭州310032

出　　处：《饮料工业》2014年第2期24-26,共3页Beverage Industry

摘　　要：建立了保健食品中姜黄素的高效液相色谱测定方法。用乙醇替代甲醇对固体颗粒样品和液体样品进行超声提取，具有一定的环保意义，以ZORBAXSB-C18（4．6mm×250mm，5μm）为分析柱，乙腈：4％乙酸-M8：52为流动相，流速为1．0mL／min，检测波长为430nm，姜黄素在0．264105．6mg／L范围内呈良好线性关系，相关系数为0．9999，检出限为0．03mg／L，相对标准偏差（n=7）3．73％，回收率90．3％-98．2％。本方法操作简便、快速、易行，是保健食品中姜黄素准确而快捷的测定方法。A method was established for the determination of curcumin in health food by high performance liquid chromatography （HPLC）. Solid particle or liquid samples were extracted in ethanol instead of methanol through ultrasonic treatment for some environmental significance. ZORBAX SB-C18 （4.6mm×250mm,5μm） was used as the analysis column, and acetonitrile-4% acetic acid solution （48 ： 52 ） as the mobile phase. The flow rate was 1.0mL/min, and the DAD detector was set at 430nm. A good linear relationship was obtained between the curcumin concentrations of 0.264- 105.6mg/L, r = 0.99999, the detect limit was 0.03mg/L, and RSD（n= 7） was 3.73%. The recovery of curcumin was in the range of 90.3%-98.2%. The method is simple, accurate and reliable, and can be used for the determination of curcumin in health food.

关 键 词：高效液相色谱法 姜黄素 保健品 

分 类 号：O657.72[理学—分析化学]