P-ERK1/2和Ki-67在胃黏膜病变中的表达及其意义  

Expression of P-ERK1/2 and Ki-67 and its significance in gastric mucosa pathological changes

作　　者：郭凤英[1] 张建中[1] 胡尚平[1] 常越[1] 

机构地区：[1]宁夏医科大学病理学系,宁夏银川750004

出　　处：《宁夏医学杂志》2014年第2期105-107,I0001,共4页Ningxia Medical Journal

基　　金：宁夏科技攻关计划项目(20101685)

摘　　要：目的探讨P-ERK1/2和Ki-67在胃黏膜病变中的表达及临床意义。方法选取胃黏膜活检标本124例,其中轻度慢性萎缩性胃炎20例、轻度慢性萎缩性胃炎伴肠上皮化生与非典型增生21例,中度慢性萎缩性胃炎24例、中度慢性萎缩性胃炎伴肠上皮化生及非典型增生23例和胃癌36例。采用S-P免疫组织化学法检测P-ERK1/2和Ki-67蛋白在不同胃黏膜活检病变组织中的表达情况。结果①P-ERK1/2在轻度慢性萎缩性胃窦炎、轻度慢性萎缩性胃炎伴肠上皮化生与非典型增生和中度慢性萎缩性胃炎中均为阴性,中度慢性萎缩性胃炎伴肠上皮化生与非典型增生及胃癌组织中的表达呈递增趋势,且各组间差异有统计学意义(P<0.05)。②Ki-67在轻度慢性萎缩性胃窦炎中为阴性表达,在轻度慢性萎缩性胃炎伴肠上皮化生及非典型增生和中度慢性萎缩性胃炎中表达分别为66.67%、79.17%,在胃癌组织中表达为83.33%,而在中度慢性萎缩性胃炎伴肠上皮化生及非典型增生阳性表达为56.52%,且各组间差异有统计学意义(P<0.05)。③Ki-67蛋白与P-ERK1/2蛋白的表达呈正相关(P<0.05)。结论 P-ERK1/2蛋白可能参与细胞的恶性转化促进了胃癌的发生发展;Ki-67蛋白与P-ERK1/2蛋白的表达呈正相关,提示两者可能共同参与了胃癌的发生和演变过程。Objective To explore the expression of P - ERK1/2 and Ki - 67 in gastric mucosa lesions. Methods Expression of P - ERK1/2 and Ki - 67 were examine in 124 cases of gastric mucosa biopsy. And there were 20 cases of the mild chronic atrophic gastritis, 21 cases of the mild chronic atrophic gastritis with intestinal metaplasia and atypical hyperplasia, 24 cases of moderate chronic atrophic gastritis, 23 cases of moderate chronic atrophic gastritis with intestinal metaplasia and atypical hyperplasia and 36 cases of gas tric carcinoma by immunohistochemical technique. Results The negative expression of P - ERK1/2 in mild chronic atrophic gastritis, mild chronic atrophic gastritis with intestinal metaplasia and atypical hyperplasia and moderate chronic atrophic gastritis. The expression of P - ERKI/2 were showed a trend of increasing in the moderate chronic atrophic gastritis with intestinal metaplasia and atypical hyper- plasia and gastric cancer tissue. The differences were statistically signifieant between the groups （ P 〈 0.01 ）. Negative expression of Ki -67 protein in mild chronic atrophic gastritis. The expression of Ki -67 was 66.67% and 79.17% in mild chronic atrophic gastritis with intestin metaplasia and atypieal hyperplasia and moderate chronic atrophic gastritis. The positive expression was 83.33% in gastric cancer tissue. The positive expression was 56.52% in moderate chronic atrophic gastritis with intestinal metaplasia and atypical hy perplasia. The differences were statistically significant between the groups （P 〈 0.01 ）. The expression of Ki -67 protein was positively correlated with that of P - ERK1/2 protein （P 〈 0.01 ）. Conclusion P - ERK1/2 protein involves in the malignant cell transformation to promote the oeeurrence of gastric cancer development. A significant positive relationship is observed between the expression of Ki - 67 and P- ERK1/2. They contribute to the carcinogenesis and develooment zastric carcinoma in coordination.

关 键 词：胃癌 P-ERK1 2 KI-67 慢性萎缩性胃炎 非典型增生 

分 类 号：R735.2[医药卫生—肿瘤]