骨髓自然杀伤细胞水平对急性髓细胞性白血病患者预后的影响  

The effect of natural killer cells level in bone marrow on the prognosis of patients with acute myeloid leukemia

作　　者：董毅[1,2] 朱太岗[1,2] 张飞虎[1,2] 汤继春[1,2] 夏瑞祥[3] 曾庆曙[3] 葛健[3] 

机构地区：[1]皖北煤电集团总医院 [2]蚌埠医学院第三附属医院血液科,安徽宿州234011 [3]安徽医科大学第一附属医院血液科,安徽合肥230022

出　　处：《蚌埠医学院学报》2014年第2期204-206,共3页Journal of Bengbu Medical College

基　　金：蚌埠医学院科研基金资助项目(Byky1264NF)

摘　　要：目的:探讨骨髓自然杀伤(natural killer,NK)细胞水平对急性髓细胞性白血病(acute myeloid leukemia,AML)患者预后的影响。方法:研究分成AML初治组40例,缓解组20例,正常对照组20例。均抽取骨髓液2 ml,采用直接免疫荧光标记法对骨髓有核细胞进行单克隆抗体(CD16+/CD56+)标记,应用流式细胞仪进行NK细胞数量的检测。结果:初治组患者骨髓NK细胞表达水平较缓解组显著降低(P<0.01)。缓解组患者骨髓NK细胞表达水平仍低于对照组(P<0.05)。不同骨髓NK细胞表达水平间原始细胞数量和AML患者疗效差异均有统计学意义(P<0.01和P<0.05)。结论:骨髓NK细胞表达水平可以作为AML的预后指标,亦有助于指导治疗。Objective：To explore the effects of natural killer（NK） cells in bone marrow on the prognosis of patients with acute myeloid leukemia（AML）. Methods：Sixty patients with AML were divided into original treating group （40 cases） and remission group （20 cases）,20 health people were served as control group. The CD16 ＋/CD56 ＋ of monoclonal antibodies in bone marrow nucleated cells of three groups were detected by direct immunofluorescence labeling method, the levels of NK cells were determined with flow cytometry. Results：The levels of NK in original treating group were significantly lower than those in remission group（P 〈 0.01 ） ,the levels of NK in remission group were significantly lower than those in normal group（ P 〈 0.05 ）. The original cell number in different NK cell levels and the differences of curative effect in AML patients were statistically significant（ P 〈 0.01 and P 〈 0.05 ）. Conclusions： The level of NK cells in bone marrow is a prognostic index of AML and can help to guide treatment.

关 键 词：急性髓细胞性白血病 自然杀伤细胞 预后 

分 类 号：R733.712[医药卫生—肿瘤]