一项关于食品中空肠弯曲菌计数方法的研究  

A research on the method of enumeration campylobacter jejuni in foods

作　　者：栾军[1] 蒋原[1] 唐泰山[1] 周阳[2] 祝长青[3] 顾其芳[3] 乔昕[4] 

机构地区：[1]江苏出入境检验检疫局,江苏南京210001 [2]安徽农业大学茶与食品科技学院,安徽合肥230000 [3]上海市疾病预防控制中心,上海200000 [4]江苏省疾病预防控制中心,江苏南京210000

出　　处：《口岸卫生控制》2014年第1期34-38,共5页Port Health Control

基　　金：国家标准制定项目:NO.20073794-T-424

摘　　要：目的 为了了解食品中空肠弯曲菌的数量,探寻一种实用的空肠弯曲菌计数方法.方法 通过不同浓度的染菌实验,对平板计数法、MPN法和Simplate方法进行比较分析.结果 当染菌量在10 CFU/g（mL）时,MPN法中至少有1管出现了阳性,simplate也可以检出且与设定值接近.因此这两种方法的检测限均为≥10 CFU/g（mL）.平板计数法的检测限应为≥30 CFU/g（mL）,在此范围内得到的结果与设定值相差0.9 ～2.4倍,小于10倍.结论 事实上,实验室可以选用不同的方法来评价食品中空肠弯曲菌的污染情况.如果检疫过程中要求结果准确,我们建议使用MPN和Simplate方法来计数加工过的食品.对于猪肉和家禽这类高污染的畜产品,选用平板计数法计数则更便捷.Objective We are seeking for a useful enumeration method of the quantity of campylobacter jejuni in foods. Methods We compared methods of plate count, MPN and Simplate by analyzing samples which have been spiked with different levels of positive culture. Results At least 1 tube of MPN results turned out to be positive at a spiked level of 10 CFU/g （mL）. The Simplate results are also close to set values. Therefore the sensitivity of the two methods are all greater than or equal to 10 CFU/g （mL）. The sensitivity of plate count method is no less than 30 CFU/g （mL）. Within this range, the results are 0.9 -2.4 times （less than 10 times） of the set values. Conclution In fact different methods can be chosen to evaluate food contaminated with campylobacter jejuni. If inspection results need to be accurate, we recommend using MPN and Simplate method to enumerate processed food. At the same time plate count method is recommended for high contaminated food such as pork and poultry.

关 键 词：空肠弯曲菌 平板计数 MPN Simplate 

分 类 号：R155[医药卫生—营养与食品卫生学]