HPV感染、宫颈炎、CIN及宫颈癌患者的宫颈黏膜脱落细胞端粒酶定量研究  

The cervical mucosal exfoliated cells telomerase quantitative research in HPV infection, cervicitis,CIN and cervical cancer patients

作　　者：傅祝能[1] 吴荣辉[1] 孙海芳 王贤芳[1] 孙兰青[1] 陈林海 

机构地区：[1]浙江义乌市中心医院,322000 [2]浙江义乌市妇幼保健院

出　　处：《中华实验和临床病毒学杂志》2014年第1期23-25,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：浙江义乌市科技局科研资助项目[2011]编号11-3-05

摘　　要：目的 检测研究表明女性在感染人乳头瘤病毒（HPV）后引起宫颈炎、宫颈上皮内瘤变（CIN）及进而发展为宫颈癌时其宫颈黏膜脱落细胞端粒酶活性量发生变化,探其对筛查和早期诊治女性宫颈癌患者的临床价值.方法 选HPV检测阴性的正常对照组26例、HPV阳性组28例、HPV阳性宫颈炎组27例、HPV阳性CIN组26例、宫颈癌组24例、HPV阴性宫颈炎组26例、HPV阴性CIN组23例共七个检测组,采集宫颈黏膜脱落细胞标本以293细胞为阳性标准进行端粒酶TRAP实时荧光定量检测.结果 端粒酶活性定量值：正常对照组,范围0～3.15、平均0.36；HPV阳性组,范围0～21.6、平均1.98,＞10的3例；HPV阳性宫颈炎组,范围0～71.5、平均4.88,＞10的4例；HPV阳性CIN组,范围0～ 407、平均20.8,＞10的5例；宫颈癌组,范围1.2 ～5.5＆#215;106、中位数346,其中＜10的4例；HPV阴性宫颈炎组,范围0～66.6、平均4.19,＞10的3例；HPV阴性CIN组,范围0 ～158、平均14.2.统计学分析宫颈癌组与其他各组比较差异有统计学意义（P＜0.01）,HPV阳性CIN组与正常对照组比较有统计学意义（P＜0.05）,其余各组间两两比较无统计学意义（P＞0.05）.结论 女性在感染HPV并引起宫颈炎、CIN阶段,其宫颈黏膜脱落细胞端粒酶活性逐步增高,在宫颈癌阶段增高最为显著,因此对采取宫颈黏膜脱落细胞进行端粒酶活性定量检测,可作为对女性宫颈癌的有效筛查和早期诊断手段之一.Objective Studies show that women infected with human papillomavirus （HPV）induced cervicitis,cervical intraepithelial neoplasia （CIN） and on to develop cervical cancer their cervical mucosal exfoliated cells telomerase activity changes,to explore its clinical value in screening and early diagnosis and treatment of female patients with cervical cancer.Methods Elections of HPV testing negative control group 26 cases,HPV-positive group 28 cases,HPV-positive cervicitis group of 27 cases,HPV-positive CIN group 26 cases,24 cases of cervical cancer,HPV-negative cervicitis group 26 cases,HPV-negative CIN group of 23 cases of seven test group,cervical mucosa cytology specimens collected in 293 cells as positive standards for real-time fluorescence quantitative detection of telomerase TRAP Quantitative value of telomerase activity.Results Normal control group,ranging from 0 to 3.15,average 0.36; HPV-positive group,ranging from 0 to 21.6,with an average 1.98,〉 10,3 cases; HPV-positive cervicitis group,ranging from 0 to 71.5,with an average 4.88,〉 10,4 cases; HPV-positive CIN group,ranging from0 to 407,with an average 20.8,〉 10,5 cases; cancer group,the range 1.2-5.5 ＆#215; 106,median 346,where 〈 10.4 cases; HPV-negative cervicitis group,ranging from 0 to 66.6,with an average 4.19,〉 10,3 cases; HPV-negative CIN group,ranging from 0 to 158,with an average 14.2.Statistical analysis of cervical cancer group and the other groups are statistically significant （P 〈0.01）,HPV-positive CIN group compared with the control group was statistically significant （P 〈 0.05） the rest of the comparison between two groups was not statistically significance （P 〉 0.05）.Conclusions Women infected with HPV and cause cervicitis,CIN stage,the cervical mucosa telomerase activity gradually increased and in cervical cancer stage increased the most significant.And therefore take cells exfoliated cervical mucosa quantitative detection of telomerase activity can be used as effective method in screening and early diagn

关 键 词：端粒末端转移酶 宫颈疾病 乳头状瘤病毒 人 

分 类 号：R737.33[医药卫生—肿瘤]