三七总皂苷的抗糖尿病机理及其降血糖成分研究  

Major Hypoglycemic Ingredients of Panax Notoginseng Saponins for Treating Diabetes

作　　者：钟振东[1] 王春梅[2] 汪为[2] 申理[2] 陈重华[2] 

机构地区：[1]四川省医学科学院.四川省人民医院实验动物研究所,成都610021 [2]四川大学华西药学院药理教研室,成都610041

出　　处：《四川大学学报（医学版）》2014年第2期235-239,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的采用遗传性2型糖尿病KK-Ay鼠探讨三七总皂苷(PNS)的降糖、减肥作用机理及其降血糖的主要有效成分。方法空腹血糖(FBG)≥13.89mmol/L的KK-Ay鼠分别注射PNS(200mg/kg体质量),人参皂苷Re(14mg/kg体质量)、人参皂苷Rd(15mg/kg体质量)、人参皂苷Rg1(40mg/kg体质量)、人参皂苷Rb1(60mg/kg体质量),三七皂苷R1(6mg/kg体质量)及生理盐水(10mL/kg体质量,模型组),共7组,每组6只,以健康C57鼠为正常对照(6只,10mL/kg体质量生理盐水)。正常对照、模型及PNS组腹腔注射给药30d,人参皂苷各组及三七皂苷R1组腹腔注射给药12d,测定FBG、糖耐量、血清胰岛素、胰岛素抵抗指数(IRI)、瘦素、体质量、摄食量、脂肪组织及血脂水平。结果 PNS、Rb1组12d时FBG较模型组下降(P<0.05),余各实验组FBGL与模型组比较差异无统计学意义(P>0.05)。PNS连续给药30d后,FBG较模型组进一步降低(P<0.01),而血清胰岛素水平(P<0.05)和IRI(P<0.01)也明显下降;体质量增长率、摄食量、脂肪质量均较模型组降低(P均<0.05),瘦素水平也较模型组下降(P<0.05)。结论 PNS通过提高KK-Ay鼠对胰岛素和瘦素的敏感性,发挥抗高血糖、抗肥胖作用,Rb1可能为PNS提取物中的降血糖成分。Objective To explore the mechanism of Panax notoginseng saponins （PNS） on diabetes treatment and mass loss in KK-Ay mice with genetic type 2 diabetes mellims （DM）, and to identify the main hypoglycemic ingredients. Methods C57 and KK-Ay DM mice were divided into eight groups each comprising six mice： healthy normal, DM model, and DM model treated with PNS （200 mg/kg body mass）, ginsenoside Re （Re, 14 mg/kg body mass）, ginsenoside Rd （Rd, 15 mg/kg body mass）, ginsenoside Rgl （Rgl, 40 mg/kg body mass）, ginsenoside Rbl （Rbl, 60 mg/kg body mass） and notoginsenoside R1 （R1, 6 mg/kg body mass）. The PNS were intraperitoneal injection administered for 30 d, while the Re, RB1, Rgl, Rd and Re were intraperitoneal injection administered for 12 d. The fasting blood sugar （FBG）, glucose tolerance （GT）, serum insulin, leptin, body weight, food consumption, and levels of adipose tissue and blood lipid were determined. Results On 12 d, lower FBG levels were found in the PNS and Rbl treated mice compared with the model mice （P〈0.05）. No statistical differences in FBG levels were found between the rest of the treatment groups and the model group （P〈0. 05）. After 30 d continuous administration of PNS, the FBG level of the mice further declined （P〈0.01）. Meanwhile, the serum insulin （P〈0. 05） and insulin resistance index （P〈0. 01） of the PNS treated mice also declined significantly. Compared with model group, the PNS group had lower levels of body weight growth, food consumption, adipose tissue, and leptin （P%0.05）. Lower FBG level was also found in Rbl treated mice （12 d of administration）, P〈0.05. Conclusion PNS has anti-hyperglycemic and anti-obesity activities by improving insulin and leptin sensitivities in KK-Ay mice. Rbl may be the hypoglycemic ingredient in the PNS extract.

关 键 词：三七总皂苷(PNS) 降血糖成分 人参皂苷RB1 抗糖尿病 机理 

分 类 号：R259[医药卫生—中西医结合]