胃癌组织TS及TUBB3表达临床意义分析  

Expression and clinical significance of TS and TUBB3 in gastric cancer tissues

作　　者：徐振宇[1] 陈海涛 茆勇[1] 华东[1] 

机构地区：[1]无锡市第四人民医院肿瘤内科,江苏无锡214062 [2]盐城市第一人民医院病理科,江苏盐城224001

出　　处：《中华肿瘤防治杂志》2014年第5期372-376,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：江苏省自然科学基金(BK2012538)

摘　　要：目的:探讨人胃癌组织中胸苷酸合成酶(thymidylate synthase,TS)与β微管蛋白Ⅲ(βⅢ-tubulin,TUBB3)的表达,及其与胃癌术后辅助化疗敏感性及预后的关系。方法:免疫组织化学SP法检测2006-01-01-2007-12-31在无锡市第四人民医院接受手术治疗且术后行辅助化疗的216例胃癌组织中TS及TUBB3的表达,统计分析与患者的临床病理特征、术后无病生存期(disease-free time,DFS)及总生存时间(overall survival,OS)的关系。结果:TS阳性表达定位于细胞质,部分表达于细胞核。在胃癌组织中的阳性表达率为49.0%(106/216),在印戒细胞癌中的表达明显高于其他病理类型,r=-0.167,P=0.014;其表达随分化程度的升高而降低,r=-0.142,P=0.038。与性别、年龄和分化程度等其他临床病理因素无明显相关性,P>0.05。TUBB3阳性表达定位于细胞质,在胃癌组织中的阳性表达率为37.5%(81/216),与性别、年龄和病理类型等临床病理因素均无明显相关性,P>0.05。TS阳性表达组的DFS为21.9个月,明显低于阴性表达组的32.4个月,χ2=5.274,P=0.022。TUBB3阳性表达组的DFS为22.5个月,明显低于阴性表达组的33.1个月,χ2=8.789,P=0.003。TS及TUBB3阳性表达组的OS均有低于阴性表达组的趋势,但差异无统计学意义,P>0.05。结论:TS和TUBB3的表达可作为预测胃癌术后紫杉醇联合氟尿嘧啶类的辅助化疗疗效较为有价值的指标,对判断预后无明确的参考价值。OBJECTIVE：To observe the expression of thymidylate synthase （TS） and βⅢ-tubulin （TUBB3） in gas- tric cancer tissues and find the association of TS and TUBB3 with sensitivity of postoperative chemotherapy and progno- sis. METHODS： The expression of TS and TUBB3 in 216 gastric tumor specimens was detected by immunohistochemis- try. The associations between the expression of biomarkers and clinicopathologic variables as wells as disease free time （DFS） and overall survival （OS） were analyzed statistically. RESULTS： TS was mainly expressed in the cytoplasm, partly in the nucleus. The positive expression rate of TS was 49.0% （106/216） which was higher in Signet ring cell carcinoma than those in other types （r=- 0. 167, P = 0. 014） and significantly correlated with the degree of differentiation （r= -0. 142, P=0. 038）. DFS in TS positive patients was 21.9 months, significantly shorter than 32.4 months in TS negative patients （;（2= 5. 274,P= 0. 022）. TUBB3 was mainly expressed in the cytoplasm. The positive expression rate of TUBB3 was 37.5 % （81/216） which was not correlated with gender, age, histological types of differentiation （P〉0.05）. DFS in TS positive patients was 22.5 months also significantly shorter than 33.1 months in TS negative patients （x2 = 8. 789, P=0. 003）. OS in TS or TUBB3 positive patients was not shorter significantly than that in TS or TUBB3 negative pa- tients （P〉0.05）. CONCLUSIONS： The expression of TS and TUBB3 are both detected in gastric cancer. They are valua- ble predictions for paclitaxel/fluorouracil chemotherapy in postoperative gastric cancer patients, but may not be valuable of prognostic factors for gastric cancer patients.

关 键 词：胃癌 胸苷酸合成酶 β微管蛋白Ⅲ 化疗敏感性 预后 

分 类 号：R735.2[医药卫生—肿瘤]