肾癌患者手术及免疫治疗前后的血清蛋白指纹图谱的研究  

Study on the serum proteomic fingerprint in pre-post immunotherapy patients with renal cell carcinoma

作　　者：高燕华[1,2] 高春芳[2] 王道元[2] 赵光[2] 李宝辉[2] 邹永强[2] 

机构地区：[1]第二军医大学研究生院研二队,上海200433 [2]解放军第150中心医院泌尿外科,河南洛阳471031

出　　处：《临床泌尿外科杂志》2014年第3期191-195,共5页Journal of Clinical Urology

摘　　要：目的:分析肾癌患者手术前后及免疫治疗前后的血清蛋白指纹图谱,构建并评估血清差异蛋白诊断模型。方法:首先应用弱阳离子磁珠技术富集肾癌患者手术前后、免疫治疗后及健康对照组的血清蛋白,其次用基质辅助激光解离-飞行时间质谱(MALDI-TOF-MS)技术构建肾癌患者不同时期的蛋白指纹图谱,再应用有监督以及无监督的化学计量学模式识别方法对构建的指纹图谱进行效力评价,最后应用受试者工作特征曲线(ROC)寻求区别各个时期肾癌患者血清中的差异蛋白。结果:在无监督模式识别方法(主成分分析,PCA)结果不理想的情况下,对比了不同有监督模式识别方法构建的指纹图谱模型,结果表明遗传算法(GA)对各个时期的肾癌患者均有较高的识别率,达到了97.7%;术前和术后差异蛋白只有一个,而术前和免疫治疗后、术后和免疫治疗后存在较多的差异蛋白。结论:肾癌患者经过免疫治疗后,血清蛋白质和术前及术后存在明显差异,肾癌应用遗传算法构建的血清蛋白指纹图谱可有效识别术前、术后、免疫治疗后以及正常对照组的血清差异蛋白。Objective.. To analyze the serum proteomic tingerprmt in pre-post lmmunotnerapy panents with renal cell carcinoma and to built and evaluate the diagnosis model of the serum differentially expressed proteins. Method：Firstly, weak cationic magnetic beads technique was used to enrich the serum proteins of patients with re- nal cell carcinoma during the period of preoperation, postoperation, post-immunotherapy respectively and in the healthy control group. Secondly, matrix-assisted laser desorption-ionization time of flight mass spectrometry （MALDI-TOF MS） was used to set up the different periods of proteomic fingerprint of renal cell carcinoma pa- tients. Thirdly, effectiveness of the construction of the fingerprint was evaluated by supervised and unsupervised stoichiometric model. Lastly, receiver-operating characteristic （ROC） curve was applied to finding the difference of the serum proteins among different historical periods. Result：When the results of unsupervised pattern recogni- tion （principal component analysis PCA） method was not ideal, we compared the fingerprint model that built with different supervised pattern recognition methods. The results showed that genetic algorithm （GA） for various pe- riods of renal cell carcinoma patients had higher recognition rate （97.7 ~）, and the serum differentially expressed protein appeared only one during the period of preoperation and postoperation. But there were a lot of serum dif- ferentially expressed proteins during the period of preoperation and post-immunotherapy and during the period of postoperation and post-immunotherapy. Conclusion： The serum proteins were obviously different among different periods of preoperation, postoperation, post-immunotherapy in renal cell carcinoma patients. The serum proteomic fingerprint that built by the genetic algorithm used in renal cell carcinoma could effectively identify the serum dif- ferentially expressed protein during the period of preoperation, postoperation, post-immunotherapy and in the healt

关 键 词：肾细胞癌 免疫治疗 蛋白指纹图谱 

分 类 号：R737.11[医药卫生—肿瘤]