MMP-9与VEGF在成人肝血管瘤中的表达及其意义  

Expression and significance of matrix metalloproteinase-9 and vascular endothelial growth factor in hepatic hemangiomas of adults

作　　者：王兆京[1] 张丹丹[2] 陈志伟[1] 陈彻[1] 姚学权[1] 张峰[3] 刘福坤[1] 

机构地区：[1]江苏省中医院消化肿瘤外科,江苏南京210029 [2]江苏省省级机关医院呼吸科,江苏南京210024 [3]南京医科大学第一附属医院肝脏移植中心,江苏南京210029

出　　处：《东南大学学报（医学版）》2014年第1期66-70,共5页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:探讨MMP-9、VEGF、FLT1、KDR、FLT4在成人肝血管瘤中的表达及其临床意义。方法:收集成人肝血管瘤组织30例、正常肝组织15例,RT-PCR检测MMP-9、VEGF、FLT1、KDR、FLT4的mRNA表达,免疫组化法检测肝组织标本中MMP-9和VEGF的表达,蛋白质印迹法测定MMP-9蛋白的表达,明胶酶谱法检测标本中MMP-9和pro-MMP9的活性变化。结果:肝血管瘤中MMP-9 mRNA的表达量明显高于正常组织,VEGF、FLT1、KDR、FLT4的mRNA表达未见明显差异;免疫组化的检测结果与mRNA的趋势一致;肝血管瘤中MMP-9蛋白的表达及活性亦较正常组织明显增高。结论:MMP-9在成人肝血管瘤组织中高表达可能与基质降解重塑、内皮细胞迁移及管状结构形成有关。Objective： To address the expression and clinical significance of matrix metalloproteinase-9 （MMP- 9）, vascular endothelial growth factor （VEGF）, FLT1, KDR and FLT4 in adults with hepatic hemangiomas. Methods： Thirty hepatic hemangioma patients and fifteen healthy subjects were included in this study. The mRNA levels of MMP- 9, VEGF, FLT1, KDR and FLT4 were examined by polymerase chain reaction- based methods. Location and distribution of MMP-9 and VEGF protein expression were determined by immunohistochemistry. The activities of MMP-9 and pro-MM9 were examined by zymography and protein levels by Western blotting. Results： We observed a significant increase in tissue MMP-9 mRNA and protein levels in hepatic hemangioma compared to normal liver tissue. The activities of MMP-9 and pro-MM9 were also markedly elevated. However, no differences in mRNA and protein levels of VEGF were seen in hepatic hemangiomas of adults Conclusion： extracellular compared to normal subjects. Based on these findings, we propose that the elevation of MMP-9 is possible associated with matrix degradation, endothelial cell migration and tube formation.

关 键 词：肝血管瘤 成人 基质金属蛋白酶-9 血管内皮生长因子 

分 类 号：R575.29[医药卫生—消化系统]