液相色谱法同时测定美容美发工作场所空气中12种芳香胺  

Simultaneous determination of 12 arylamines in air of hairdresser salon workplaces by HPLC

作　　者：陈若虹[1] 阮小林[2] 林佐侃[2] 凌东辉[1] 关皑菁[1] 吴琪[1] 

机构地区：[1]广州市海珠区疾病预防控制中心,广东广州510288 [2]广东省职业病防治院,广东广州510300

出　　处：《中国卫生检验杂志》2014年第4期472-475,共4页Chinese Journal of Health Laboratory Technology

基　　金：广州市海珠区科技计划项目(2012-y1-11)

摘　　要：目的建立同时测定美容美发工作场所空气中12种芳香胺染料成分的溶剂解吸—液相色谱方法。方法工作场所空气中的芳香胺采用硅胶管采集,用10%乙腈的水溶液(pH=3.5)解吸后进样,通过液相色谱—二级阵列管检测器(HPLC—DAD)分离测定。结果该法中12种芳香胺有较好的分离度,线性相关系数在0.9990~1.0000之间,线性范围在0.5 mg/L^200 mg/L至2 mg/L^200 mg/L之间,平均相对标准偏差(RSD)为1.6%~8.3%,最低检出质量浓度为0.02 mg/m3~0.12 mg/m3(以采集12 L工作场所空气计),平均解吸效率为91.7%~100%,采样效率为92.4%~100%,100 mg硅胶管对12种芳香胺的穿透容量为643.7μg^1143μg,样品在4℃避光冷藏条件下可保存3 d。结论该法操作简便,准确度、精密度及解析效率均满足要求,适用美容美发工作场所空气中多种芳香胺的测定。Objective To establish a method for simultaneous determination of 12 arylamine dyes in air of hairdresser salon workplaees by solvent desorption - HPLC. Methods The arylamines in air were eolleeted by silieone tubes and desorbed by pH3.5 water containing 10% aeetonitrile, then the desorption solution was analyzed by HPLC - DAD. Results The 12 aryl- amines were separated well with a correlation coefficient of 0.9990 - 1. 0000, the linear range was from 0.5 mg/L ~ 200 rng/L to 2 mg/L - 200 mg/L. The average RSDs were 1.6% - 8.3% , the lowest limits of eoneentration were 0.02 mg/m3 0.12 mg/m3 with a gas volume of 12 L, the average desorption effieieneies were 91.7% - 100% , the sample eolleefion effi- eieneies were 92.4% - 100%, the penetration eapaeities of 100 mg silicone tube for the arylamines were 643.7 μg - 1143 μg. The samples could be stored for 3 days at 4 ℃ refrigerator avoiding light. Conclusion This method is simple, fast and reliable with good aceuraey and desorption efficiency for determination of multiple arylamines in workplace air of hairdresser salons.

关 键 词：芳香胺 染发剂 工作场所 空气 液相色谱法 

分 类 号：O657.72[理学—分析化学]