HPLC法检测不同温度和时间对蜂王浆中癸烯酸的影响  

Determination of 10-hydroxy-2-decenoic acid in royal jelly at different temperature and time by high performance liquid chromatography

作　　者：张娟[1] 邹丽君[1] 谭婷[2] 

机构地区：[1]赣南医学院预防医学系,赣州341000 [2]南昌大学分析测试中心,南昌330047

出　　处：《食品科技》2014年第3期270-272,共3页Food Science and Technology

基　　金：2008年赣南医学院人才引进项目(2008129)

摘　　要：采用高效液相色谱法，对不同贮藏温度和时间的蜂王浆中10-羟基-2-癸烯酸（10-HDA）含量进行测定。样品采用95％乙醇溶解，采用C18柱，流动相为v（甲醇）：v（0．1％甲酸）=55：45，流速0．5mL／min，检测波长210nm，采用外标定量法进行测定，10-HDA在质量浓度为50-400mg／L范围内呈良好线性关系，相关系数R=0．9996。研究表明：该方法简便、快速、准确、可靠，在不同温度和时间下蜂王浆中癸烯酸含量较稳定。High performance liquid chromatography was used for the determination of 10-hydroxy-2- decenoic acid in royal jelly at different temperature and time. Sample was extracted by 95% ethanol, chromatographic separation was performed using C18 column with methanol and 0.1% formic acid （v/ v, 55：45） as mobile phase at a flow rate of 0.5 mL/min. The UV detection wavelength was fixed at 210 nm, external calibration method was used in this experiment the linearity was obtaied over the range of 50~400 mg/L （R=0.9996）. The results showed that this method is simple, quick, specific and accurate, and 10-hydroxy-2-decenoic acid was stable at different temperature and time.

关 键 词：高效液相色谱 蜂王浆 10-羟基-2-癸烯酸 

分 类 号：TS207.3[轻工技术与工程—食品科学]