BIOCOCKTAIL对乳腺癌细胞MDA-MB-231凋亡的促进作用  

Effect of BIOCOCKTAIL on apoptosis of human mammary cancer cells

作　　者：张瑞山[1] 李翔[1] 谢贤鑫 李欢[1] 张腾[1] 马利艳[1] 徐宏[1] 张斌[1] 

机构地区：[1]辽宁省肿瘤医院乳腺外科,辽宁沈阳110042

出　　处：《中国微生态学杂志》2014年第2期148-150,共3页Chinese Journal of Microecology

基　　金：国家自然科学基金资助项目(81102029;81172047)

摘　　要：目的从细胞凋亡方面观察BIOCOCKTAIL(益生菌微生物制剂)对乳腺癌细胞MDA-MB-231的抑制作用。方法采用FITC和PI双染色法及TUNEL法检测凋亡。结果通过FITC和PI双染色法,观察到BIOCOCKTAIL可显著增加乳腺癌细胞MDA-MB-231凋亡细胞。Ⅰ组乳腺癌细胞系(8.1%vs 38.7%,P<0.05),Ⅱ组乳腺癌细胞系(6.9%vs 19.4%,P<0.05)。TUNEL法检测MDA-MB-231细胞凋亡现象明显增多。结论 BIOCOCKTAIL能够有效诱导乳腺癌细胞MDA-MB-231凋亡,有可能成为乳腺癌治疗的补充方案。Objective To observe the effect of BIOCOCKTAIL on apoptosis of MDA-MB-231 cells. Methods MDA-MB-231 cell apoptosis was observed by using annexin V-fluorescein isothiocyanate （FITC） and propidium io- dide （PI） double staining method and TUNEL method. Results BIOCOCKTAIL had induced MDA-MB-231 cell apoptosis. Groug I breast cancer cell（ 8.1% vs 38.7 % , P 〈 O. 05 ）. Groug Ⅱ breast cancer cells （ 8.1% vs 38. 7% , P 〈0.05）. The similar phenomenon was observed in TUNEL method. There were few apoptosis cells in the control group. Conclusion BIOCOCKTAIL can significantly induce the apoptosis of MDA-MB-231 cells.

关 键 词：BIOCOCKTAIL 乳腺肿瘤 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]