血管内皮生长因子A、基质金属蛋白酶9在WHO Ⅱ级脑膜瘤中的表达及意义  

Expression and significance of vascular endothelial growth factor A and matrix metalloproteinase 9 in WHO grade Ⅱ meningiomas

作　　者：王大鹏[1] 沈晓黎[1] 何伟[1] 涂斌锋[2] 吴雷[1] 张焱[1] 祝新根[1] 

机构地区：[1]南昌大学第二附属医院神经外科,330006 [2]江西省肿瘤医院头颈外科

出　　处：《临床神经病学杂志》2014年第1期12-15,共4页Journal of Clinical Neurology

基　　金：国家自然科学基金项目(30760258);江西省科技厅科技计划项目(20123BBG70239;20133BBG70072);江西省卫生厅科技计划(20063083)

摘　　要：目的研究血管内皮生长因子(VEGF)-A、基质金属蛋白酶(MMP)-9在WHOⅡ级脑膜瘤中的表达及意义。方法分别采用Western blot法和反转录聚合酶链反应(RT-PCR)检测17例WHOⅡ级脑膜瘤患者、37例WHOⅠ级脑膜瘤患者及5例脑外伤患者的脑组织VEGF-A、MMP-9蛋白和mRNA的表达,并进行比较。结果 WHOⅡ级组各型VEGF-A、MMP-9蛋白及mRNA表达显著高于对照组和WHOⅠ级组各型(P<0.05)。WHOⅠ级组各型VEGF-A、MMP-9蛋白及mRNA表达显著高于对照组(均P<0.05)。结论 VEGF-A、MMP-9蛋白和mRNA在WHOⅡ级脑膜瘤中的表达显著升高,可能促进了WHOⅡ级脑膜瘤的侵袭和复发。Objective To study the expression and significance of vascular endothelial growth factor A （VEGFA） and matrix metalloproteinase 9 （MMP-9） in WHO grade Ⅱ meningiomas.Methods Expression of VEGF-A,MMP-9 protein and mRNA in brain tissue in 17 WHO grade Ⅱ meningiomas patients,37 WHO grade Ⅰ meningiomas patients and 5 patients with traumatic brain injury was detected by Western blot and reverse transcription PCR.The results were compared.Results Expression of VEGF-A,MMP-9 protein and mRNA in brain tissue in each types of WHO grade Ⅱ meningiomas group were significantly higher than those in control group and each types of WHO grade Ⅰ meningiomas group （all P 〈 0.05）.Expression of VEGF-A,MMP-9 protein and mRNA in brain tissue in each types of WHO grade Ⅰ meningiomas group were significantly higher than those in control group （all P 〈 0.05）.Conclusion The expression of VEGF-A,MMP-9 protein and mRNA in WHO Ⅱ grade meningiomas are significantly elevated,which may promote the invasiveness and recurrence of WHO grade Ⅱ meningiomas.

关 键 词：血管内皮生长因子A 基质金属蛋白酶9 WHOⅡ级脑膜瘤 

分 类 号：R739.45[医药卫生—肿瘤]