串珠素在大鼠牙髓损伤修复过程中的时空表达  

Temporal and spacial expression of perlecan in dental pulp after injury

作　　者：李桥[1] 孙虹[2] 潘乙怀[2] 刘传通[3] 

机构地区：[1]温州医科大学附属口腔医院儿童口腔科,浙江温州325027 [2]温州医科大学附属口腔医院牙体牙髓病科,浙江温州325027 [3]温州医科大学附属口腔医院口腔种植科,浙江温州325027

出　　处：《上海口腔医学》2014年第1期15-19,共5页Shanghai Journal of Stomatology

基　　金：浙江省教育厅科研计划项目(20051196);温州市科技计划项目(Y20100015)

摘　　要：目的:研究大鼠牙髓损伤修复过程中串珠素的时空表达变化及作用机制。方法 :选用24只雄性SD大鼠,通过暴露一侧大鼠上颌磨牙牙髓建立牙髓损伤模型,对侧未损伤磨牙作为对照组,术后1、3、5、7 d取标本,脱钙后石蜡包埋,近远中向沿牙体长轴4μm切片,采用免疫组织化学染色观察串珠素和β1整合素的表达变化,平均吸光度法计算相对表达量,采用SPSS19.0软件包对数据进行单因素方差分析。结果:与对照组相比,实验组串珠素染色强度增加,阳性染色位置随着炎症损伤的进展方向而转移,各组平均光密度均高于对照组(F=9.511,P<0.05),并以1 d组升高最为明显。β1整合素染色在牙髓损伤后3 d开始,在炎症牙髓和相对正常牙髓中阳性表达,3、5、7 d组的平均吸光度均显著高于对照组(F=124.18,P<0.05)。结论:串珠素在牙髓组织损伤后防御机制中可能通过调节血管生成和炎症细胞浸润而发挥作用。PURPOSE： To investigate the temporal and special expressions and functions of perlecan in dental pulp of rats after injury. METHODS： Specimens of one side of maxillary molars of 24 male SD rats were collected 1,3,5,7 days after injury, and the other side were used as controls. The specimens were decalcified and embedded in paraffin, sliced by longitudinal section and stained by immunohistochemistry. The staining of perlecan and β1 integrin were examined, and the average optical density （AOD） was compared. The measurement data were analyzed by ANOVA using SPSS 19.0 software package. RESULTS： The staining intensity of perlecan in dental pulp in the experimental group was significantly higher compared with that of the control group. The immunolocalization of perlecan in dental pulp was gradually progressed as the inflamed tissue was progressing after injury, and its AOD was significantly higher than that of control group （F=11.104,P〈0.05）, especially in the group of 1 d. The staining of β1 integrin was generally enhanced in the pulp 3 d after injury, and its AOD was significantly higher than that of control group （F=124.18,P〈0.05）. CONCLUSIONS： Perlecan may play an important role in tissue defense mechanism after injury through regulating angiogenesis and inflammatory cell infiltration. Supported by Science and Technology Research Project of Department of Education of Zhejiang Province （20051196） and Wenzhou City （Y20100015）.

关 键 词：串珠素 B1整合素 免疫组化 大鼠 牙髓损伤 

分 类 号：R781.3[医药卫生—口腔医学]