SAMD9在食管鳞癌中的表达及其意义  

Significance of SAMD9 expression in esophageal squamous cell carcinoma

作　　者：汤善宏[1] 郑琇山[2] 贺莉[1] 乔丽娟[1] 景丹[1] 倪阵[1] 曾维政[1] 蒋明德[1] 

机构地区：[1]成都军区总医院消化内科 [2]成都军区总医院胸外科

出　　处：《细胞与分子免疫学杂志》2014年第4期411-413,416,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：成都军区总医院研究型人才资助项目(2013YG-B027)

摘　　要：目的研究SAMD9(sterile alpha motif domain-containing 9)在食管癌中表达和临床意义。方法选取72例手术切除食管癌及癌旁组织,采用免疫组织化学染色法检测SAMD9在食管癌及癌旁组织表达差异,并分析其在食管癌组织表达临床意义。另外选取3例手术时已发生转移及3例手术时未发现任何转移的食管癌组织,Western blot法检测SAMD9在2组中表达差异。结果 SAMD9在食管癌组织及癌旁食管鳞状上皮细胞中表达无明显差异;SAMD9在食管癌组织中表达水平与患者淋巴管浸润及淋巴结转移密切相关(P<0.05),而与患者性别、年龄、分化及T分期无明显相关性(P>0.05)。Western blot法结果显示SAMD9在转移性食管癌组织中表达水平显著强于非转移性食管癌组织(P<0.01)。结论 SAMD9在食管癌组织中表达水平与转移密切相关。Objective To analyze the significance of sterile alpha motif domain-containing 9 （SAMDg） expression in esophageal squamous cell carcinoma （.ESCC）. Methods Immunohistochemical staining was performed to detect the expression of SAMD9 in 72 primary ESCC and matched adjacent cancer-free tissues and analyze the significance of SAMD9 expression in ESCC tissues. In addition, Western blotting was used to detect the expression of SAMD9 in primary ESCC tissues which were taken from 3 metastatic and 3 non-metastatic patients during surgery. Results The expression of SAMD9 in the ESCC tissues had no statistical difference from that in the matched adjacent cancer-free tissues. SAMD9 expression was significantly correlated with lymphatic invasion and metastasis in these patients （P 〈 0.05 ）, but not with age, gender, tumor differentiation and T stage （ P 〉 0.05 ）. Western blotting showed that SAMD9 expression in primary ESCC tissues was significantly higher in the patients with metastasis than in the ones without metastasis（P〈0. 01）. Conclusion Over-expression of SAMD9 is correlated with the metastasis of ESCC, indicating that it might play an important role in the metastasis of ESCC.

关 键 词：SAMD9 食管癌 转移 

分 类 号：R735.1[医药卫生—肿瘤] R392.33[医药卫生—临床医学]