LETM1蛋白在结肠癌组织中的表达及临床意义  

Clinical significance of LETM1 protein expression in colonic cancer

作　　者：高爱花[1] 金永民[1] 孙红花[1] 金文彪[1] 崔海[1] 崔现[1] 崔玉珍[1] 沈雄虎[1] 张松男[1] 朴龙镇[1] 

机构地区：[1]延边大学附属医院肿瘤科,吉林省延吉市133000

出　　处：《世界华人消化杂志》2014年第5期718-723,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;Nos.81060166;81160270;81160275;高等学校博士学科点专项科研基金资助项目;No.20102201120003~~

摘　　要：目的:观察亮氨酸拉链EF-hand结构域跨膜蛋白1(leucine zipper/EF-hand-containing transmembrane protein 1,LETM1)在结肠癌中的表达情况,探讨LETM1蛋白是否能够成为判断结肠癌患者预后的新的生物学指标.方法:利用免疫组织化学法检测73例结肠癌患者组织中LETM1蛋白的表达水平和蛋白激酶B(protein kinase B,PKB)/Akt的丝氨酸473(Ser473)、苏氨酸308(Thr308)两个磷酸化位点及葡萄糖合成激酶3β(glycogen synthase k i n a s e 3β,G S K3β)的磷酸化水平,并根据LETM1蛋白表达水平进行高表达和低表达分组;采用Kaplan-Meier法分析各组无疾病生存期;采用χ2检验进行组间单因素分析.结果:在结肠癌患者中LETM1蛋白高表达率为54.8%,LETM1蛋白在结肠癌浸润深度、分化及淋巴结转移中低表达和高表达率分别为27.3%/72.7%、38.3%/61.7%和22.7%/77.3%,其差异均有统计学意义(P<0.05),而与年龄、性别、肿瘤大小未见统计学意义(P>0.05).LETM1蛋白在结肠癌组织中可以激活磷脂酰肌醇3激酶(phosphatidylinositol 3 kinase,PI-3K)/A k t信号传导通路.L ETM1高表达患者无疾病生存期显著低于LETM1低表达患者(P<0.05).结论:LETM1蛋白在结肠癌中高表达,可能参与结肠癌的发生、发展与复发.LETM1蛋白可以作为预测结肠癌患者预后的新的生物学指标.AIM： To observe the expression of leucine zip- per/EF-hand-containing transmembrane pro- tein 1 （LETM1） protein in colonic cancer and to determine whether LETM1 can be used as a marker for this malignancy. METHODS： Immunohistochemical method was used to detect the expression of LETM1 and phosphorylated Akt （protein kinase B, PKB/ Akt） and glycogen synthase kinase 3β （GSK3β）in 73 specimens of colonic carcinoma. Disease- free survival time of colonic cancer patients was analyzed by Kaplan-Meier method. Univariate analysis was performed to determine the correlation between LETM1 pathologic parameters of expression and clinicocolonic cancer. RESULTS： The rate of high LTEM1 protein expression was 54.8% in 73 colonic cancer specimens. LETM1 protein expression was significantly correlated with degree of invasion （27.3% vs 72.7%, P 〈 0.05）, differentiation （38.3% vs 61.7%, P 〈 0.05） and lymph node metastasis （22.7% vs 77.3%, P 〈 0.05）, but not with age, sex or tumor size （P 〉 0.05 for all）. High expression of LETM1 protein could activate the phosphatidylinositol 3 kinase （PI3K）/Akt signal transduction pathway. The disease-flee survival time of patients with high expression of LETM1 was significantly lower than that of patients with low expression of LETM1 （P 〈 0.05）. CONCLUSION： High expression of LETM1 may play a role in the pathogenesis, metastasis and recurrence of colon cancer. LETM1 protein may be used as an indicator to determine the prognosis of colon cancer patients.

关 键 词：亮氨酸拉链EF—hand结构域跨膜蛋白1 结肠癌 蛋白激酶B 

分 类 号：R735.35[医药卫生—肿瘤]