血管内皮钙黏蛋白在非小细胞肺癌中的表达及其意义  

Expression and significance of vascular endothelial cadherin in non-small cell lung cancer

作　　者：周俊豪[1] 陶韬[1] 陈虹[1] 顾雪霜[1] 彭单伊[1] 张丽君[1] 

机构地区：[1]重庆医科大学附属第一医院呼吸与危重症医学科,重庆400016

出　　处：《重庆医科大学学报》2014年第1期17-21,共5页Journal of Chongqing Medical University

基　　金：重庆市自然科学基金资助项目(编号:cstc2011jjA10052)

摘　　要：目的:探讨血管内皮钙黏蛋白(vascular endothelial cadherin,VE-cadherin)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及其与临床病理特征之间的关系,并分析其与血管内皮生长因子(vascular endothelial grow factor,VEGF)之间的相关性。方法:应用免疫组化SP法检测72例NSCLC组织及相应癌旁正常组织VE-cadherin的表达情况;其中34例用qRTPCR法检测mRNA的表达情况,并以VEGF为阳性参照。Western blot检测肺癌细胞株A549和人正常支气管上皮细胞(normal human bronchial epithelial cells,NHBEC)中VE-cadherin的表达,以及干扰后各细胞株的表达情况。结果:72例NSCLC组织癌细胞和VE-cadherin的阳性表达率分别为69.4%、52.8%,均明显高于对应癌旁正常组织肺泡上皮细胞0%和血管内皮细胞20.8%(P<0.05);有淋巴结转移组中的表达量均明显高于无淋巴结转移组(P<0.05);Ⅲ、Ⅳ期的阳性表达率均明显高于Ⅰ、Ⅱ期(P<0.05)。VEGF在NSCLC组织癌细胞和血管内皮细胞中的阳性表达率分别为84.7%、62.5%,均显著高于对应的癌旁正常组织肺泡上皮细胞16.7%和血管内皮细胞29.2%(P<0.05)。经qRT-PCR分析,VE-cadherin mRNA在癌组织和癌旁正常组织中的相对表达量分别为1.15±0.87、0.70±0.57;VEGF mRNA的相对表达量分别为1.39±1.17、0.79±0.65(P<0.05)。两者在蛋白水平和mRNA水平均呈正相关(P=0.001,P=0.008)。Western blot结果示在A549细胞株中可检测到VE-cadherin表达,但在NHBEC中不表达。siRNA可降低VE-cadherin在A549细胞株中的表达。结论:VE-cadherin可能在NSCLC血管生成中起重要作用,且可能与VEGF具有协同作用,siRNA可有效抑制VE-cadherin在肺癌细胞株A549中的表达。Objective：To investigate the expression of vascular endothelial cadherin （VE-cadherin） in non-small cell lung cancer （NSCLC） and its association with the clinicopathological characteristics and vascular endothelial grow factor（VEGF）. Methods ：Ex- pression of VE-cadherin was examined at protein level by immunohistochemical staining in 72 cases of NSCLC tissues and their ad- jacent non-neoplastic tissues;34 of which were measured at mRNA level by qRT-PCR and VEGF was taken as positive reference. Expression of VE-cadherin in lung cancer cell line A549 and normal human bronchial epithelial cells（NHBEC） was detected by Western blot and VE-cadherin gene expression was inhibited with siRNA in A549 cells. Results：Positive rates of VE-cadherin in cancer cells and vascular endothelial cells were 69.4% and 52.8%, which were higher than those in alveolar epithelial ceils （0%） and vascular endothelial cells（20.8%） in adjacent non-neoplastic tissues（P〈0.05）. Positive rates of VE-cadherin were higher in cases with lymph node metastasis than in cases without lymph node metastasis（P〈0.05）. Positive rates of VE-cadherin were higher at m and Ⅳ stage than at I and Ⅱ stage（P〈0.05）. Positive rates of VEGF in cancer cells and vascular endothelial cells were 84.7% and 62.5%,which were higher than those in alveolar epithelial cells（16.7%） and vascular endothelial cells（29.2%） in adjacent non-neo- plastic tissues （P〈0.05）. qRT-PCR results demonstrated that relative expression of VE-cadherin mRNA in NSCLC tissues and adjacent non-neoplastic tissues were 1.15 ±0.87 and 0.70± 0.57;relative expression of VEGF mRNA were 1.39± 1.17 and 0.79± 0.65（P〈 0.05）. VEGF and VE-cadherin were positive correlated at both protein and mRNA levels（P=0.001 ,P=0.008）. Western blot demon- strated that VE-cadherin expression could be detected in the A549 cells,but not in NHBEC and sequence-specific siRNA can sig- nificantly decreased expression of VE-cadherin in A549 cells. Conclusion

关 键 词：非小细胞肺癌 血管内皮钙黏蛋白 血管生成 RNA干扰 

分 类 号：R734.2[医药卫生—肿瘤]