高脂饲料喂养时间和STZ剂量对建立2型糖尿病大鼠模型的影响  

Effects of High-fat Diet Feeding Time and Streptozotocin Dose on Establishment of a Rat Model of Type 2 Diabetes Mellitus

作　　者：魏占英[1] 沈丽[1] 冯晓慧[1] 孙文广[1] 葛声[1] 

机构地区：[1]上海市第六人民医院营养科,200233

出　　处：《医学研究杂志》2014年第2期42-46,共5页Journal of Medical Research

基　　金：中国医师协会临床营养发展基金资助项目(CN2012017)

摘　　要：目的探索高脂饲料喂养时间及STZ剂量对制备2型糖尿病大鼠模型及其血糖稳定性的作用。方法 60只雄性SD大鼠随机分为6组:正常对照组(CN组)普通饲料喂养;模型1组(M1组)HFD喂养4周+STZ35mg/(kg·bw)腹腔注射;模型2组(M2组)HFD喂养4周+STZ38mg/(kg·bw)腹腔注射;模型3组(M3组)HFD喂养8周+STZ25mg/(kg·bw)腹腔注射;模型4组(M4组)HFD喂养8周+STZ30mg/(kg·bw)腹腔注射;模型5组(M5组)HFD喂养10周+STZ25mg/(kg·bw)腹腔注射。STZ注射后观察大鼠空腹血糖(FBG)、空腹胰岛素(FINS)、甘油三酯(TG)、胆固醇(TC)、口服糖耐量实验(OGTT)及血糖曲线下面积(AUC)变化。结果模型组FBG、FINS、TG、TC、OGTT各点血糖及AUC均显著高于正常对照组(P<0.01)。随着高脂喂养时间和STZ注射剂量的增加2型糖尿病大鼠成模率和死亡率均呈上升趋势,M2组成模率高达到90%,但死亡率同样高达50%,M1和M4组成模率分别为70%和80%,死亡率低于M2。本研究各模型组2型糖尿病大鼠血糖在造模后第2周能够达到稳定状态。结论 40%HFD喂养8周联合30mg/(kg·bw)STZ腹腔注射建立的2型糖尿病动物模型具有成模率高、死亡率低及血糖稳定等特点,是一种值得推广的造模方法。建议使用造模后第2周大鼠空腹血糖判断是否造模成功。Objective To investgate the effects of different high - fatdiet feeding time and STZ doses on the establishment of a rat model of type 2 diabetes mellitus and the stability of blood sugar. Methods Sixty male SD rats were divided into 6 groups randomly：nor- mal controlgroup（NC） ; model 1 group（ M1 ）, high - fat diet（ four weeks） ＋ STZ35mg/（ kg ~ bw） intraperitoneal injection ; model 2 group （ M2 ）, high - fat diet （ four weeks） ＋ STZ38mg/（ kg · bw） ; model 3 groups （ M3 ）, high - fat diet （8 weeks） ＋ STZ25mg/（ kg · bw） ; model 4 group（M4） ,high - fat diet（8 weeks） ＋ STZ30mg/（ kg · bw） ; model 5 groups（MS） ,high - fat diet（ 10 weeks） ＋ STZ25mg/ （kg · bw）. We observed the fasting blood glucose（FBG） , fasting insulin（FINS） , triglycerides（TG） , cholesterol（TC） , oral glucose tol- erance test（OGTT） and glucose area under curve （AUC） of the rat model of type 2 diabetes after STZ injected. Results The FBG, FINS, TG, TC, blood glucose of OGTT and AUC of model groups were significantly higher than those of control group （ P 〈 0.01 ）. As the high -fat feeding time and the injection dose of STZ increasing,the type 2 diabetes model rates and mortality rates were all increased. M2 had the highestmodel rates and mortality rate, respectively as 90% and 50%. The model rats of M1 and M4 were respectively as 70% and 80% and compared with M2 the mortality rate was lower. The FBG of type 2 diabetes animal model in this study would reach a steady state in 2 weeks after STZ injection. Conclusion Forty percent HFD feeding 8 weeks with 30mg/（kg · bw） STZ is a kind of modeling method to be promoted. Type 2 diabetesanimal model established by this method had characteristics of high model rate, low mortality rate and a stable blood sugar. It is recommended to use the fasting glucose of 2 week after STZ injection to determine whether getting a successful model.

关 键 词：2型糖尿病 高脂饲料 链脲佐菌素 大鼠 模型 

分 类 号：R58[医药卫生—内分泌]