骨肉瘤CD133 CD117 Ki-67的表达及与临床病理因素和危险度的相关性研究  

Expression of CD133,CD117,and Ki-67 in human osteosarcoma and their clinical significance

作　　者：林金銮[1] 林建华[1] 吴朝阳[1] 蓝文彬[2] 李祥[2] 刘蔚楠[3] 冯进益[2] 王发圣[1] 

机构地区：[1]福建医科大学附属第一医院骨科,福州市350005 [2]福建医科大学第一临床学院 [3]福建中医药大学附属人民医院骨科

出　　处：《中国肿瘤临床》2014年第5期305-310,共6页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金专项基金(编号:81241087);福建医科大学临床医学重点学科(编号:XK201106)资助~~

摘　　要：目的:研究骨肉瘤组织CD133、CD117及Ki-67在蛋白水平的表达情况及其与临床病理因素和危险度的关系。方法:应用免疫组织化学PV-9000二步法检测55例骨肉瘤组织标本中CD133、CD117和Ki-67的表达,并与临床病理学指标和术后无瘤生存期进行比较分析,对照组为20例骨软骨瘤。应用SPSS 17.0软件统计分析,检验标准为P<0.05具有统计学意义。结果:骨肉瘤组织CD133、CD117、Ki-67蛋白表达阳性率显著高于良性的骨软骨瘤组织,差异有统计学意义(分别为P=0.016、P=0.008、P<0.001);CD133或Ki-67蛋白阳性表达骨肉瘤患者平均生存时间及平均转移时间短于CD133或Ki-67蛋白阴性骨肉瘤患者,差异有统计学意义(P<0.05);CD133、Ki-67在外科分期和远处转移对骨肉瘤患者预后有影响,其中CD133是外科分期及远处转移而影响骨肉瘤患者预后的独立因素。结论:CD133和Ki-67表达可能在骨肉瘤发生、发展过程中起重要作用,有望成为判断其预后的指标。Objective： This study aims to investigate the protein expression of CD 133, CD 117, and Ki-67 in human osteosarcoma tissues and explore their relationships with the clinico-pathological features and risk of osteosarcoma. Methods： Immunohistochemical method was used to examine the protein expression of CD 133, CD 117, and Ki-67 gene in the paraffin specimens of 55 and 20 cases of osteosarcoma and osteochondroma, respectively. SPSS 17.0 statistical software was used to explore the relationships among the expressions of CD 133, CD117, and Ki-67 gene and the biological behavior and prognosis of osteosarcoma. Test criterion： P〈0.05 was considered statistically significant. Results： The positive expression rates of CD 133, CD 117, and Ki-67 were significantly higher in the osteosarcoma tissues than in the benign osteochondroma tissues, and the differences were significant （P=0.016, P--0.008, and P〈0.001, respectively）. The mean survival and metastasis time were shorter in the CD 133 or Ki-67 positively expressed osteosarcoma patients than in those with CD 133 or Ki-67 negatively expressed osteosarcoma. The differences were significant （P〈0.05）. The expression of CD 133 and Ki-67, surgical staging, and distal metastasis were relevant to the prognosis of osteosarcoma patients. The expression of CD133 and distal metastasis are the independent risk factors that affect the prognosis of these patients. Conclusion： The positive expressions of CD 133 and Ki-67 closely correlated with the occurrence and progression of osteosarcoma, and may be used as an indicator for prognosis of the cancer.

关 键 词：骨肉瘤骨 肉瘤干细胞 CDL33 CDll7 KI-67 

分 类 号：R738.1[医药卫生—肿瘤]