DHM对3-NP诱导的PC12细胞损伤的保护作用  

Protective Effect of DHM on 3-nitropropionic acid induced oxidative stress in PC12 cells

作　　者：李幽兰[1] 杨川[1] 欧阳丽斯[1] 马宇昕[2] 贾钰[1] 陈思[1] 邬加佳[1] 穆淑花[1] 刘冰冰[1] 雷万龙[1] 

机构地区：[1]中山大学中山医学院人体解剖学教研室,广东广州510080 [2]广东药学院基础学院人体解剖学教研室,广东广州510080

出　　处：《解剖学研究》2014年第1期23-26,共4页Anatomy Research

基　　金：国家自然科学基金(31070941;30770679);国家科委973项目(2010CB530000)分题(2010CB530004)

摘　　要：目的研究二氢杨梅素(DHM)对3-硝基丙酸(3-nitropropionic acid,3-NP)诱导的PC12细胞损伤的保护作用及可能机制。方法 MTT法测定细胞存活率,TBA(硫代巴比妥酸)法检测丙二醛(MDA)含量,WST-1(水溶性四唑盐)法检测超氧化物歧化酶(SOD)活性,实验数据采用SPSS17.0软件统计分析。结果①MTT结果显示3-NP处理能显著降低PC12细胞的存活率,而DHM预处理对此变化有抑制作用;②MDA测定结果显示3-NP处理能显著增加PC12细胞的MDA含量(P<0.05),而与3-NP处理组相比,3-NP+DHM组PC12细胞的MDA含量显著降低(P<0.05),接近对照组水平(P>0.05);③SOD测定结果显示,与对照组比较,3-NP处理显著降低了PC12细胞SOD的活性(P<0.05),而3-NP+DHM组PC12细胞SOD的活性较3-NP组有显著提高(P<0.05),接近对照组水平(P>0.05)。结论②DHM预处理能拮抗3-NP诱导的PC12细胞的损伤,其机制可能与降低细胞内脂质过氧化水平,提高抗氧化酶的活性有关。Objective To investigate the protective effect and the possible mechanism of dihydromyricetin （DHM） on in- jury induced by 3-nitropropionic acid in PC12 cells. Methods HD model was induced by addition of 3-NP into PC12 cells for 24h. PC12 cells were pretreated with different doses of DHM to explore the optimum concentration of the best protective effect. Cell viabil- ity, the level of malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） were measured by MTI＂ assay, the method of thiobarbituric acid reaction and the method of water-soluble tetrazolium salt reaction. Results MTF assay showed that 3- NP dose-dependently decreased cell viability and the 24 h LCS0 for 3-NP in PC12 cells was 8 mmol/L. Cell viability was signifi- cantly increased by DHM at the concentration of 100 ttmol/L and 10 Ixmol/L. Further, DHM treatment significantly downregulated. the level of MDA in PC12 ceils increased by 3-NP （P 〈 0.05） and also restored the activity of SOD in PC12 cells which was pro- foundly lowered by 3-NP （P 〈 0.05）.

关 键 词：二氢杨梅素 3-硝基丙酸 PC12细胞 氧化应激 

分 类 号：R742.2[医药卫生—神经病学与精神病学]