白灵菇高活力液体菌种摇瓶发酵培养条件的优化  

The Optimization of the High Energy Liquid Culture Conditions of Pleurotus nebrodensis

作　　者：董玉兰[1] 李书生[1] 张丽萍[1] 程辉彩[1] 习彦花[1] 张根伟[1] 尹淑丽[1] 

机构地区：[1]河北省科学院生物研究所,河北石家庄050081

出　　处：《北方园艺》2014年第6期143-146,共4页Northern Horticulture

基　　金：河北省科技攻关资助项目(09235502D);河北省科学院科技计划资助项目(11326)

摘　　要：以白灵菇为试材,通过摇瓶方法,以白灵菇菌丝生物量、菌球密度及萌发活力指数为检测指标,对白灵菇高活力液体菌种培养条件进行了优化研究,以期提高白灵菇液体菌种的活力。结果表明:该菌株的最适培养条件为温度28℃,转速180r/min,培养时间60h;最佳培养基配方为3.0%玉米粉、1.5%葡萄糖、0.3%蛋白胨、2.0%黄豆粉、0.1%KH2PO4、0.1%MgSO4。Taking Pleurotus nebrodensis as material ,the appropriate liquid fermentation medium were studied by measuring the mycelial biomass, the peloton density and the amount of germination vigor index through shake flask method to enhance the vitality of Pleurotus nebrodensis strains of liquid. The results showed that the optimum culture conditions were as below：the culture temperature was 30℃,the optimal shaking frequency was 180 r/min and training time was 60 h. The optimum medium were 3. 0% of the corn flour, glucose 1. 5 %, peptone 0. 3 %, soybean meal 2. 0 %, KH2 PO4 0. 1% ,MgSO4 0. 1%.

关 键 词：液体菌种 培养条件 生物量 菌球密度 萌发活力 

分 类 号：S646[农业科学—蔬菜学]