不同施氮量烤烟氮素代谢差异及其对香气前体物形成的影响  

Flue-cured Tobacco Nitrogen Metabolism Differences and Its Influence on Aroma Precursors in Different Amount of Nitrogen

作　　者：李飞[1] 杨铁钊[1] 张小全[1] 武云杰[1] 李丽华[1] 康雪莉[1] 符新妍 宋洋洋[1] 

机构地区：[1]河南农业大学烟草学院,河南郑州450002

出　　处：《华北农学报》2014年第1期170-177,共8页Acta Agriculturae Boreali-Sinica

基　　金：中国烟草总公司特色优质烟叶开发重大专项(Ts-01-2011003)

摘　　要：为了探索烤烟品种氮素代谢差异对烟叶香气前体物形成的影响,运用大田施肥试验,测定了不同施氮水平下豫烟10号和中烟203成熟过程中的氮素代谢产物和相关酶活性的变化,同时测定了类胡萝卜素和多酚含量。结果表明,品种间GS、GDH等氮素代谢相关酶活性不同决定了氮素转化方式和转移规律,是导致基因型间氮素代谢差异的重要因素。类胡萝卜素和多酚含量与氮代谢产物和相关酶活性的相关性分析:在生长成熟前期类胡萝卜素含量与GS的相关系数达0.515*,在成熟后期与GS、GDH的相关系数分别达0.664**和0.645**;在整个成熟期多酚含量与NR的相关系数达到-0.524**。品种间比较说明烤烟成熟前期氮代谢旺盛有利于类胡萝卜素的形成,成熟后期氮代谢强度减弱有利于类胡萝卜素降解产物的形成;烤烟成熟期NR活性低,硝酸盐积累则多酚的形成受到抑制。In order to explore the differences of nitrogen metabolism in varieties and its influence on tobacco a-roma precursors formation. Under the condition of the field,measured nitrogen metabolites and related enzyme activ-ity in different varieties under different nitrogen levels, at the same time determined carotenoids and polyphenols content. The results indicated that the difference of enzymes related to nitrogenous metabolism such as GS,GDH in varieties,determined the way of nitrogen transformation and the rule of the nitrogen transfer. It is an important factor that lead to nitrogen metabolism differences of genotype. Correlation analysis showed that grow mature early,correla-tion coefficient between carotenoid content and GS were 0. 515＊;grow mature late,correlation coefficient between carotenoid content and GS,GDH were 0. 664＊＊and 0. 645＊＊;In the mature polyphenol content and NR correlation coefficient reaches -0. 524＊＊. By maturity of flue-cured tobacco,the NR activity was low,nitrate accumulation,the formation of polyphenols is restrained.

关 键 词：烤烟 氮代谢 类胡萝卜素 多酚 

分 类 号：S572[农业科学—烟草工业]