微小RNA-29a对人胃癌细胞裸鼠移植瘤生长的影响  

The effects of microRNA-29a on the growth of human gastric cancer cells transplanted subcutaneously in nude mice

作　　者：陈陵[1] 王宗华[2] 宋航[1] 黄一[1] 王国威[1] 王仙园[2] 任辉[2] 

机构地区：[1]解放军第324医院消化内科,重庆400020 [2]解放军第三军医大学护理学院

出　　处：《中华实验外科杂志》2014年第3期474-476,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30900679）;重庆市自然科学基金资助项目（cstc2011jjA10111）

摘　　要：目的 观察微小RNA（miR）-29a对人胃癌SGC-7901细胞裸鼠皮下移植瘤生长的影响.方法 以磷酸盐缓冲液（PBS）为空白对照,以负载cel-miR67的复制缺陷型腺病毒（Ad-cel）为阴性对照,注射负载miR-29a的复制缺陷型腺病毒（Ad-miR29a）液于移植瘤内,观察移植瘤生长曲线和抑瘤率;采用实时定量逆转录聚合酶链反应（RT-qPCR）检测移植瘤内miR-29a表达丰度;采用免疫组织化学技术检测CD34表达以计算移植瘤内微血管密度（MVD）.结果 与PBS组比较,Ad-miR29a组移植瘤内miR-29a表达显著上调（3.06±0.73比1.00±0.21,P＜0.01）,而Ad-cel组与PBS组比较差异无统计学意义（P＞0.05）;Ad-miR29a组移植瘤生长速度低于PBS组,而Ad-cel组与PBS组差异无统计学意义（P＞0.05）;于第5周末Ad-miR29a组抑瘤率达49.30％;Ad-miR29a组的移植瘤内MVD显著低于PBS组（21.3±8.1比42.6±11.2,P＜0.01）,而Ad-cel组与PBS组比较差异无统计学意义（P＞0.05）.结论 miR-29a可显著抑制裸鼠胃癌移植瘤生长,其机制可能为抑制移植瘤内MVD,miR-29a有望成为新型的胃癌基因治疗靶标.Objective To study the effect of microRNA （miR）-29a on the growth of human gastric cancer cell line SGC-7901 xenografts in nude mice.Methods The SGC-7901 cell line was injected subcutaneously into nude mice to establish the model of human gastric cancer xenografts.The replicationdeficient recombinant adenovirus carrying miR-29a （Ad-miR29a） was injected into the xenografts.The phosphate buffered saline （PBS） solution was employed as blank control and the adenovirus carrying celmiR-67 （Ad-cel） as negative control.The growth curve of the tumor xenografts was observed and the inhibitory rate of tumor was calculated.The expression of miR-29a was detected using real-time reverse transcriptase-polymerase chain reaction （RT-qPCR）.The microvessel density （MVD） was calculated by CD34 detection using immunohistochemistry in the xenografts.Results Comparing to the PBS group,the expression level of miR-29a of xenografts in the Ad-miR29a group was significantly up-regulated （3.06 ± 0.73 vs.1.00 ±0.21,P ＜ 0.01）,while no significant difference was detected between Ad-cel and PBS group.The growth of xenografts in the Ad-miR29a group was suppressed apparently comparing to the PBS group,while no significant change was detected between Ad-cel and PBS group.At the end of the 5th weekend,the tumor inhibition rate reached 49.30％ in Ad-miR29a group.The MVD counts of xenografts in the Ad-miR29a group were down-regulated significantly comparing to that in PBS group （21.3 ± 8.1 vs.42.6 ± 11.2,P ＜0.01）,while no significant change was detected between Ad-cel and PBS group.Conclusion The up-regulation of miR-29a can suppress the growth of gastric cancer xenografts in nude mice by inhibiting the MVD in tumors,which makes miR-29a a promising novel target for gene therapy for human gastric cancer.

关 键 词：微RNAS 胃癌 微小RNA-29a 微血管密度 

分 类 号：R735.2[医药卫生—肿瘤]