Kiss1在胃腺癌组织中的表达及其对胃癌细胞增殖和转移的影响  

Expression of Kiss1 in gastric adenocarcinoma and its effect on proliferation and metastasis of gastric cancer cells

作　　者：樊清波[1] 刘红山[2] 秦秉玉[1] 王存真[1] 刘晗[1] 郑鹏远[3] 

机构地区：[1]郑州大学人民医院(河南省人民医院)重症医学科,450003 [2]郑州大学人民医院(河南省人民医院)肝胆胰腺外科,450003 [3]郑州大学第二附属医院消化内科

出　　处：《中华实验外科杂志》2014年第3期489-491,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 检测Kiss1在人胃腺癌及癌旁组织中的表达,探讨Kiss1对胃癌细胞增殖和转移的作用及其分子机制.方法 免疫组织化学法检测Kiss1在40例人胃腺癌及其癌旁组织中的表达.利用慢病毒载体建立Kiss1过表达胃癌SGC-7901细胞株,Western blot检测Kiss1、增殖细胞核抗原（PCNA）及基质金属蛋白酶-2（MMP-2）的蛋白表达水平;噻唑蓝（MTT）法和Transwell侵袭实验检测Kiss1过表达对胃癌细胞增殖和转移的影响.结果 Kiss1蛋白在胃腺癌及癌旁组织的阳性表达率分别为40.0％和67.5％,差异有统计学意义（x^2=4.302,P＜0.05）;与癌旁组织（1.00）比较,Kiss1 mRNA在胃腺癌组织中的相对表达量（0.52 ±0.13）明显降低（P＜0.01）;与对照组和空载组比较,Kiss1蛋白在转染组表达明显升高,而PCNA和MMP-2蛋白的表达下降,差异均有统计学意义（P＜0.01）.结论 Kiss1可能作为抑癌基因参与胃癌细胞增殖和转移.Objective To examine the expression of Kiss1 in human gastric adenocarcinomas and corresponding adjacent non-cancerous tissues （ANCT）,and further explore the effects and molecular mechanisms of Kiss1 in proliferation and metastasis of gastric cancer cells.Methods The expression of Kiss1 in 40 cases of gastric adenocarcinomas tissues and ANCT was detected by using immunohistochemistry.Human gastric cancer SGC-7901 cells in vitro were stably transfected with lentivirus-mediated overexpression of Kiss1 （Lv-Kiss1）,and the expression levels of Kiss1,proliferating cell nuclear antigen （PCNA） and metalloproteinase-2 （MMP-2） were detected by using Western blotting.The proliferative activity and metastatic capability of gastric cancer cells were assessed by methyl thiazol tetrazolium （MTT）and Transwell assays.Results The expression of Kissl protein was observed in 40.0％ gastric adenocarcinoma tissues compared with that in 67.5％ ANCT （P ＜ 0.01）,and its mRNA level （0.52 ± 0.13） was also decreased in gastric adenocarcinoma tissues compared with thaat in ANCT （1.00）.Overexpression of Kissl by Lv-Kiss1 could down-regulate the expression of PCNA and MMP-2,and inhibit proliferative activities and metastatic potential of gastric cancer cells.Conclusion Kissl may function as a tumor suppressor involved in gastric cancer cell proliferation and metastasis,suggesting a promising therapeutic target for the treatment of cancer.

关 键 词：Kiss1 胃癌 增殖 转移 

分 类 号：R735.2[医药卫生—肿瘤]