自噬对乙醇诱导的胃黏膜上皮细胞凋亡的影响  

Effect of autophagy on ethanol-induced apoptosis of gastric epithelial cells

作　　者：常伟龙[1] 帅晓明[1] 张鹏[1] 马牧原[1] 李伟[1] 尹玉平[1] 陶凯雄[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胃肠外科,武汉430022

出　　处：《中华实验外科杂志》2014年第3期505-507,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81172294）

摘　　要：目的 观察自噬在乙醇诱导胃黏膜上皮GES-1细胞凋亡中的作用.方法 体外使用100～1 600 mmol/L浓度乙醇诱导胃黏膜上皮GES-1细胞发生凋亡,并分为4组：空白对照组、乙醇组、乙醇＋雷帕霉素（RAPA）组和乙醇＋氯喹（CQ）组.流式细胞仪检测各组细胞凋亡水平,噻唑蓝（MTT）法检测各组细胞增殖程度,免疫荧光显微镜观察自噬小体,Western blot检测各组中微管相关蛋白1轻链32β（LC3）的表达水平.结果 细胞凋亡随着乙醇浓度增加而升高,自噬能有效降低这种凋亡.乙醇组细胞凋亡率[（17.78±7.86）％]明显高于空白对照组[（12.64±6.82）％]和乙醇＋RAPA组[（14.88±7.12）％],同时又显著低于乙醇＋CQ组[（21.44±8.69）％,P＜0.05].在乙醇干预下,诱导细胞自噬可以增加细胞的相对增殖活性.结论 乙醇可以诱导胃黏膜上皮GES-1细胞凋亡.促进细胞自噬可以有效降低乙醇诱导的细胞凋亡,抑制自噬可以有效增高乙醇诱导的细胞凋亡.Objective To investigate the impact of autophagy on the level of apoptosis induced by ethanol in gastric epithelial cells （GES-1） in vitro.Methods GES-1 cells were treated with ethanol at 100-1 600 mmol/L as an ethanol-induced apoptotic model.GES-1 cells were divided into 4 groups：control group,ethanol group,ethanol and rapamycin （RAPA,autophagy revulsant） group,and ethanol and chloroquine （CQ,autophagy inhibitor） group.Apoptosis rate was measured using flow cytometry.The methyl thiazol tetrazolium （MTT） assay was used to evaluate the cell proliferation.Autophagic level was measured by immunofluorescence microscopy.The expression of autophagy related proteins microtubule2associated protein 1 light chain 32β3（LC3） was detected by using Western blotting.Results Induction of autophagy could significantly reduce ethanol-induced apoptosis of GES-1 cells.Apoptosis rate in ethanol group [（17.78 ± 7.86） ％] was higher than that in control group [（12.64 ± 6.82） ％,P ＜ 0.05] and RAPA group [（14.88 ± 7.12） ％,P ＜ 0.05],but lower than the ethanol and chloroquine group [（21.44 ± 8.69） ％,P ＜ 0.05].Additionally,under the intervention of ethanol,up-regulation of autophagy could promote the cell proliferation of GES-1 cells.Conclusion Ethanol may induce the apoptosis of gastric epithelial cells.Up-regulation of autophagy protects GES-1 cells against ethanol-induced apoptosis while inhibition of autophagy may aggravate ethanol-induced apoptosis of GES-1 cells.

关 键 词：自噬 脱噬作用 胃上皮细胞 胃黏膜 

分 类 号：R363[医药卫生—病理学]