芝麻核心种质芝麻素和芝麻酚林的关联分析  

Association analysis of sesamin and sesamolin in the core sesame( Sesamum indicum L. ) germplasm

作　　者：王蕾[1] 黎冬华[1] 齐小琼[1] 张艳欣[1] 丁霞[1] 王林海[1] 危文亮[1] 高媛[1] 张秀荣[1] 

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物生物学重点开放实验室,湖北武汉430062

出　　处：《中国油料作物学报》2014年第1期32-37,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31201242);公益性专项(2012ZL041);农业部作物种质资源保护项目(NB2011-2130135-29(1))

摘　　要：选用来源我国黄河流域至长江流域8省215份芝麻核心种质材料，对其种子中芝麻素（sesamin）和芝麻酚林（sesamolin）含量进行测定，芝麻素平均值5．24mg／g，变异范围为0．88～11．05mg／g，变异系数38．56％，芝麻酚林平均值3．30mg／g，变异范围为0．93—6．96mg／g，变异系数22．68％，二者均符合正态分布，且相关分析表明两者间呈极显著正相关；采用标记一性状关联分析法，进行芝麻素和芝麻酚林与SSR、SRAP、AFLP标记的关联分析。利用GLM模型共检测到33个标记与芝麻素和芝麻酚林极显著（P〈0．01）关联，同时与两种成分显著关联的有4个；利用MLM模型共检测到8个显著关联的标记，与两种成分显著关联的分别有4个；其中SSR标记SSll82-3在两种模型中同时极显著关联到芝麻素和芝麻酚林，且解释率较高。该研究将为芝麻功能性成分遗传改良和分子标记研究奠定重要基础。In this study, a core sesame germplasm consisting of 215 accessions from 8 provinces along the Yellow River and the Yangtze River valley were used to analyze the content of sesamin and sesamolin. The results showed that the sesamin content ranged in 0.88 -11.05mg/g with an average of 5.24rag/g, and the coefficient of variation was 38.56%. The sesamolin content varied from 0.93 to 6.96mg/g with an average of 3.30rag/g, its coefficient of variation was 22.68%. The two components are correlated with each other positively and significantly, and both of them exhibited normal distribution in content. Association analysis was performed to detect the potential loci that related to the two components with SSR, SRAP, AFLP markers. By GLM model, a total of 33 loci significantly were associated with sesamin and sesamolin with 26 associated with sesamin and 8 assosiated with sesamolin respectively. In contrast, the MLM model detected fewer loci with 4 associated with sesamin and 4 associated with sesamolin respectively. Among these loci, there were 4 associated with sesamin, and 4 associated with sesamolin by the two models respectively. Locus SSI182 -3 was significantly associated with sesamin and sesamolin in the models, which may be one of the useful markers for maker - assisted selection.

关 键 词：芝麻 芝麻素 芝麻酚林 变异 关联分析 

分 类 号：S565.303[农业科学—作物学]