高效液相色谱-质谱-质谱法快速鉴定中药竹节香附的皂苷  

The rapid separation and identification of saponins from Chinese herb Anemone raddeana REGEL by RP-HPLC-MS-MS

作　　者：李顺意[1] 李紫[1] 王世敏[2] 陈勇[1] 

机构地区：[1]湖北大学生命科学学院,湖北武汉430062 [2]湖北大学化学与材料科学学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2000年第4期382-386,共5页Journal of Hubei University：Natural Science

基　　金：武汉中药现代化工程技术研究中心课题资助

摘　　要：:应用反相高效液相色谱 -电喷雾 -质谱 -质谱 (RP -HPLC -ESI -MS -MS)方法对竹节香附皂苷提取物的化学成分进行分析 .通过一步分析可以同时分离并鉴定出竹节香附中的竹节香附皂苷R2 、R3、R6、R7、R8和R9等 6种有效成分 .同时也发现一些本药材中原来没有报道的成分 .本研究结果为竹节香附及其相关药品有效成分分析和鉴定提供一种快速鉴定方法 .Chinese Folk Medicine “Zhu jie xiang fu” is the roots of Anemone raddeana REGEL. It is an important medicine used to treat rheumatism, neuralgia, cancer and other disease. This study was to rapid separate and identify the Saponins in the medicine. A reversed phase high performance liquid chromatography coupled with electrospray tandem mass spectrometry (RP-HPLC-ESI-MS-MS) techniques were employed. Alcohol extracts from the crude medicine was directly analyzed by RP-HPLC-ESI-MS-MS. By this method, six oleanane-type glycosides were isolated and identified. They were raddeanodises R 2, R 3, R 6,R 7,R 8 and R 9.

关 键 词：竹节香附 竹节香附皂苷 高效液相色谱-质谱-质谱法 中药 有效成分 鉴定 同分异构体 

分 类 号：R282.710.3[医药卫生—中药学] R284.1[医药卫生—中医学]