采用抗体芯片筛选鼻咽癌放射治疗抵抗相关的炎性因子  

Identii cation of inl ammation factors related to radioresistance of nasopharyngeal carcinoma by an antibody array

作　　者：李娇阳[1] 瞿家权[1] 易红[1] 易红梅[1] 肖志强[1] 

机构地区：[1]中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙410008

出　　处：《临床与病理杂志》2014年第1期15-21,共7页Journal of Clinical and Pathological Research

基　　金：国家自然科学基金(81230053;81172559)~~

摘　　要：目的:采用抗体芯片筛选鼻咽癌放射治疗(放疗)抵抗相关的炎性因子。方法:以放疗抵抗鼻咽癌CNE2 IR细胞与放疗敏感鼻咽癌CNE2细胞的细胞蛋白质及其细胞培养上清为样本,采用抗体芯片(AAH INF 3)比较炎性因子的表达差异,采用免疫组织化学方法检测差异炎性因子IL 8在放疗抵抗与敏感鼻咽癌组织(每组30例)中的表达。结果:炎性因子IL 8、粒细胞巨噬细胞集落刺激(granulocyte macrophage colony stimulating factor,GM CSF)和细胞间黏附分子 1(intercellular adhesion molecule 1,ICAM 1)在CNE2 IR细胞中的表达水平高于CNE2细胞,CNE2 IR细胞分泌的IL 8和GM CSF水平也高于CNE2细胞,而CNE2 IR细胞分泌的基质金属蛋白酶 2(matrixmetalloproteinase 2,TIMP 2)水平低于CNE2细胞;IL 8在放疗抵抗鼻咽癌组织中的表达明显高于放疗敏感鼻咽癌组织。结论:IL 8,GM CSF,ICAM 1和TIMP 2表达或分泌异常可能在鼻咽癌放疗抵抗中具有重要作用,为进一步研究鼻咽癌放疗抵抗的分子机制提供了新线索。Objective： To screen inflammation factors related to radioresistance of nasopharyngeal carcinoma （NPC）. Methods： Antibody array of human inflammation factors （AAH-INF-3） were used to compare the expression differences between radioresistant NPC CNE2-IR and radiosensitive NPC CNE2 cells. Immunohistochemistry was performed to detect the expression levels of differential inflammation factor IL-8 in the radioresistant and radiosensitive NPC tissues （30 cases for each group）. Results： The expression levels of IL-8, granulocyte- macrophage colony stimulating factor （GM-CSF） and intercellular adhesion molecule 1 （ICAM-1） in CNE2-IR cells were significantly higher than those in CNE2 cells, and CNE2-IR cells secreted more IL-8 and GM- CSF than CNE2 cells while secreted lesser matrixmetalloproteinase 2 （TIMP-2） than that in CNE2 cells. Immunohistochemistry showed that the expression levels of IL-8 were significantly higher in the radioresistant NPC tissues than those in the radiosensitive NPC tissues. Conclusion： Abnormal expression or secretion of IL- 8, GM-CSF, ICAM- 1, and TIMP-2 may play an important role in NPC radioresistance, which may provide a novel clue for further investigating the molecular mechanism ofNPC radioresistance.

关 键 词：鼻咽癌 放射治疗抵抗 抗体芯片 炎性因子 

分 类 号：R739.63[医药卫生—肿瘤]