银杏营养贮藏蛋白质的分离纯化与结构鉴定  

Isolation,Purification and Structural Determination of Vegetative Storage Proteins in Ginkgo biloba

作　　者：吴彩娥[1] 范龚健[1] 李婷婷[1] 吴海霞[1,2] 徐文斌[1] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037 [2]运城学院生命科学系,运城044000

出　　处：《林业科学》2014年第2期37-41,共5页Scientia Silvae Sinicae

基　　金：江苏省科技支撑(农业)项目(BE2011381);江苏省普通高校研究生科研创新计划项目(CXZZ12-0544);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：为探究银杏营养贮藏蛋白质的结构与功能的关系,采用DEAE-Sepharose FF离子交换色谱和Sephadex G-75葡聚糖凝胶色谱对银杏营养贮藏蛋白进行分离纯化,得到一条36 kDa的蛋白。质谱分析结果表明:该蛋白中的2个肽段序列与大豆贮藏蛋白质(29 kDa)和薯蓣块茎贮藏蛋白质(30 kDa)的部分氨基酸序列吻合。红外光谱结果表明:β-折叠构象是银杏营养贮藏蛋白质的主要二级结构。To reveal the relationship between the structure and function of vegetative storage proteins in Ginkgo biloba, the vegetative storage proteins were separated and purified by DEAE-Sepharose FF ion-exchange chromatography and Sephadex G-75 gel chromatography. A target protein was obtained and its molecule weight was 36 kDa. The mass- spectrometry showed that there were two peptide sequences which were consistent to some sequences of the Soybean storage protein （29 kDa） and the Dioscorea storage protein （30 kDa）. The infrared spectrometry indicated that the secondary structure of the protein was mainly in β-sheet conformations.

关 键 词：银杏 营养贮藏蛋白质 分离纯化 结构鉴定 

分 类 号：S718.43[农业科学—林学]