硼替佐米体外对前列腺癌细胞迁移和侵袭的影响及其机制  

The influence of bortezomib on migration and invasion in cultured human prostatic cancer cells and its mechanism

作　　者：胡炜[1] 王嵬[1] 高振宇[2] 

机构地区：[1]辽宁省锦州市中心医院肿瘤内科,121000 [2]辽宁省锦州市中心医院肾内科,121000

出　　处：《肿瘤研究与临床》2014年第2期98-101,共4页Cancer Research and Clinic

摘　　要：目的 探讨不同剂量的硼替佐米对前列腺癌DU145细胞迁移和侵袭的影响及其可能机制。方法 0、10、20 nmol／L硼替佐米处理DU145细胞24 h后，采用Transwell小室检测DU145细胞迁移，Matrigel侵袭实验检测细胞侵袭能力。采用Western blot技术检测黏附相关蛋白黏着斑激酶（FAK）及其自主磷酸化位点Tyr397的表达水平。结果 10、20 nmol／L硼替佐米作用24 h后，DU145细胞的迁移指数为（69.05±10.56）、（52.55±6.98）个，明显低于未经硼替佐米处理组的（81.55±10.56）个（均P＜0.05）；10、20 nmol／L硼替佐米处理组细胞侵袭指数分别为（39.35±6.45）、（32.05±4.22）个，明显低于未经硼替佐米处理组的（58.75±5.41）个（均P＜0.05）；硼替佐米处理组同时伴有Try397表达水平的下调，但FAK总蛋白的表达不受影响。结论 蛋白酶体通路抑制剂硼替佐米可能通过下调FAK Tyr397的表达，抑制前列腺癌DU145细胞迁移和侵袭能力。Objective To investigate the effects of bortezomib on cellular migration and invasion ability in cultured human prostatic cancer cells DU145 and its mechanism. Methods Transwell method was used to detect cell migration. Invasion was assayed by Matrigel-coated invasion chambers. Expressions of FAK and Tyr397 proteins were analyzed by Western blot. Results After treated by bortezomib at the concentration of 10, 20 nmol/L for 24 h, the invasion index of DU145 cells were （69.05±10.56） and （52.55±6.98）, they were gradually reduced compared with untreated group （81.55±10.56） （P 〈 0.05）. The migration index were （39.35±6.45）, （32.05±4.22）, they were also reduced compared with untreated group （58.75±5.41） （P 〈 0.05）. The group of treated by bortezomib showed Tyr397 protein expression had been suppressed. However FAK protein had not marked change. Conclusions FAK is involved in the regulation of cellular migration and invasion function. Bortezomib might inhibit cells migration and invasion function by down regulation of Tyr397 expression.

关 键 词：前列腺肿瘤 硼替佐米 细胞运动 肿瘤侵润 黏着斑激酶 

分 类 号：R737.25[医药卫生—肿瘤]