pH胁迫对法乐水绵生长指标和环境应激酶的影响  

Effects of pH stress on growth index and environment stress kinase of Spirogyra farlowii Transeau

作　　者：山鹰 王丽卿[1] 赵风斌[1,2] 徐玉萍[1] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海水生环境工程有限公司,上海200090

出　　处：《环境污染与防治》2014年第2期23-26,31,共5页Environmental Pollution & Control

基　　金：国家水体污染控制与治理科技重大专项(No.2012ZX07101-007)

摘　　要：以淡水丝状藻法乐水绵为研究对象,分别研究了在不同pH下其环境应激酶、叶绿素a含量和细胞死亡率的变化,以期能为水体中淡水丝状藻的控制提供理论依据.结果表明,在pH为6.0～8.0时,法乐水绵对pH胁迫具有一定的抗逆性,表现为叶绿素a含量和环境应激酶含量变化较平稳.但随着pH胁迫的增加,法乐水绵细胞对胁迫引起的自由基损伤修复的能力下降.法乐水绵细胞通过形成更多的环境应激酶、增加其活性等途径来消除因胁迫而生成的多余自由基、缓解对膜系统的攻击.但pH超过8.5后,抗逆性减弱,法乐水绵出现生长抑制,pH胁迫对细胞结构的破坏加剧,导致出现细胞结构变异和死亡现象.Spirogyra farlowii Transeau was selected as target object to study the effect of pH stress on its en- vironmental stress kinase, chlorophyll-a and cell death rate. The research would provide reference to the theoretical basis for the removal of freshwater filamentous algae. The results showed that in the pH range of 6.0 to 8.0, Spiro- gyra farlowii Transeau presented certain resistance to pH stress, the content of chlorophyll a and environmental stress kinase varied smoothly and increased slightly. But with the pH stress increased, the repair capacity of Spiro- gyra farlowii Transeau against damage caused by free radical would be declined. In order to reduce moisture loss and ensure the normal physiological function of cells, Spirogyra farlowii Transeau cells would secreted more environ- mental stress kinase and increased their activity to remove excess free radicals, relieve the attack to the membrane sys- tem, prevent the intensification of the membrane lipid peroxidation. While, when the pH stress exceeded the thresh- old level of 8.5, the resistance of cells would be weakened, resulting the growth inhibition, the damage on the cell structure, cell structure variation and mortality.

关 键 词：PH胁迫 法乐水绵 环境应激酶 细胞死亡率 

分 类 号：X173[环境科学与工程—环境科学] X52