miRNA-374a在乳腺癌侵袭与转移中的作用及机制  

The role of miRNA-374a in invasion and metastasis of breast cancer and its mechanism

作　　者：田瑞[1] 李建一[1] 张文海[1] 贾实[1] 张扬[1] 

机构地区：[1]中国医科大学附属盛京医院乳腺外科,110000

出　　处：《中华内分泌外科杂志》2014年第1期53-56,61,共5页Chinese Journal of Endocrine Surgery

基　　金：辽宁省科学技术计划项目（2012225016）

摘　　要：目的 探讨miRNA-374a在乳腺癌侵袭转移过程中的作用及机制.方法 选取实验标本48例,其中高侵袭转移性乳腺癌前缘组织标本（A组）、低侵袭转移性乳腺癌前缘组织标本（B组）、正常乳腺组织标本（C组）各16例.每组随机选取6例标本混合后分别进行高通量基因组学分析.筛选出差异表达的miRNA-374a.通过生物信息学工具预测miRNA-374a的靶蛋白BMP-2.运用qRT-PCR及Western blot方法检测各组剩余10例标本的miRNA-374a mRNA、BMP-2 mRNA及BMP-2蛋白表达水平以进行验证.所得数据运用SPSS 18.0统计分析.结果 miRNA-374a mRNA的表达次序为：C组＞B组＞A组（P＜0.05）,BMP-2 mRNA表达次序为：C组＜B组≈A组（P＜0.05）,BMP-2蛋白表达次序为：C组＜B组＜A组（P＜0.05）.相关性分析显示miRNA-374a mRNA与BMP-2蛋白存在明显负相关（P=-0.412 8）.结论 miRNA-374a通过调节BMP-2的蛋白表达抑制乳腺癌侵袭转移.Objective To discuss the role of miRNA-374a in invasion and metastasis of breast cancer.Methods Breast tissues from 48 patients（32 patients with breast cancer）were classified into high invasive and metastatic group（HIMG,group A）,low invasive and metastatic group（LIMG,group B）,and normal group （group C）.Each group contained 16 specimens.6 samples were selected randomly from each group and were detected by microRNA array.Key microRNA （miRNA-374a）was selected and its target protein（BMP-2） was predicted by bioinformatics software.The rest 10 specimens of each group were detected by real-time qRT-PCR and Western blot to test the content of miRNA-374a,mRNA and protein of BMP-2.Correlation analysis was performed between miRNA and the target protein.SPSS 18.0 was used to make statistical analysis.Results The relative content of miRNA-374a was higher in group C than in group B,higher in group B than in group A（P ＜ 0.05）.BMP-2 mRNA level was higher in group A and group B than in group C （P ＜ 0.05）.The expression level of BMP-2 protein was lower in group C than in group B,lower in group B than in group A（P ＜0.05）.Correlation analysis showed that miRNA-374a was negatively correlated with BMP-2 protein（P =-0.412 8）.Conclusion miRNA-374a inhibits the invasion and metastasis by regulating BMP-2 in human breast cancer.

关 键 词：乳腺癌 转移 异质性 miRNA-374a 骨形态发生蛋白-2 

分 类 号：R737.9[医药卫生—肿瘤]