PM_(2.5)暴露对大鼠行为学及器官的急性毒理作用  

Acute Toxicity of PM_(2. 5) Exposure on Rat Behavior and Organs

作　　者：李峰[1] 石辉[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《生态毒理学报》2014年第1期127-132,共6页Asian Journal of Ecotoxicology

基　　金：青年科技基金(No.QN1043)

摘　　要：通过气管滴注的方法,研究不同浓度细颗粒物(PM2.5)对运动大鼠行为学及不同器官的毒性作用,探讨PM2.5暴露对运动状态下机体的急性毒理作用及其可能的机制。将32只雄性Wistar SPF(Specific Pathogen Free,SPF)大鼠随机分为运动对照组(EC)、低剂量PM2.57.5 mg·(kg·bw)-1+运动组(LPE)、中剂量PM2.515 mg·(kg·bw)-1+运动组(MPE)、高剂量PM2.530 mg·(kg·bw)-1+运动组(HPE)。采用一次性气管滴注染毒后进行递增负荷跑台训练,利用卒中指数对运动过程中大鼠进行行为学评分,并测定大鼠肺、肝脏、股四头肌组织谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px),活性氧(reactive oxygen species,ROS),核转录因子核转录因子kappa B(nuclear factor-B,NF-κΒ)和单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)浓度。与运动对照组相比,3个暴露组中大鼠的卒中指数差异均有统计学意义(p<0.05或p<0.01);PM2.5暴露组GSH-Px下降,差异均有统计学意义(p<0.05或p<0.01);ROS,NF-κΒ和MCP-1上升差异均有统计学意义(p<0.05或p<0.01),并呈现出剂量相关性。急性PM2.5暴露可对大鼠行为学产生不利的影响,导致不同器官的抗氧化及免疫指标发生改变,影响机体的运动能力,造成某些生理损伤。The 32 male Wistar SPF（Specific Pathogen Free, SPF）rats were randomly selected and divided into blank control group （QC）, exercise control group （EC）, low dose of PM2.5 ＋ exercise group （LPE）, middle dose ofPMz~ ＋ exercise group（MPE）, high dose ofPM2.5 ＋ exercise group （HPE）. PM2.5 was administered to rats by intratracheal instillation at the doses of 7.5 mg.（kg.bw）-1, 15 mg.（kg.bw）-1 and 30 mg.（kg.bw）-1respectively, then all rats were trained by treadmill running with increasing load. Stroke index score was used to evaluate the rats ＇ behavior and the GSH-Px, ROS, NF-KB and MCP-1 were tested. The stroke index were statistically significant （p 〈0.05 orp 〈0.01）in three group compared with the control group; the activity of GSH-Px de- creased after PM2~ exposed, and the differences were statistically significant （p 〈0.05 orp 〈0.01）. The indexof ROS, NF-KB and MCP-1 was statistically increased （p 〈 0.05 or p 〈 0.01）, which showed dose-related. Acute exposure by PM2.5 can be adversely affected rats＇ behavior, resulting in some changes of the anti-oxidant and immune. Accordingly, the body＇s sports ability were affected and some physiological damage occurred.

关 键 词：细颗粒物 卒中 抗氧化酶 免疫 

分 类 号：X171-5[环境科学与工程—环境科学]