3种小麦作物二氢黄酮醇4-还原酶(DFR)基因的生物信息学分析  

Bioinformatics Analysis of Dihydroflavonol 4-reductase and the Gene in Three Triticeae Crops

作　　者：潘怡辰[1] 王坤[1] 王汝茜[1] 张云洁[1] 李集临[1] 张杰[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025

出　　处：《中国农学通报》2014年第6期72-76,共5页Chinese Agricultural Science Bulletin

基　　金：哈师大博士科研启动基金项目"黑粒小麦色素的分子遗传研究"(08XBSK87)

摘　　要：为了研究彩色小麦色素合成中相关酶的性质及其基因,对已在NCBI上注册的普通小麦、蓝粒小麦、天蓝偃麦草的二氢黄酮醇4-还原酶(dihydroflavonol-4-reductase,DFR)核酸和氨基酸序列进行分析。通过生物信息学软件研究3种作物二氢黄酮醇4-还原酶的酶学特性,对其组成成分、理化性质、亚细胞定位、跨膜结构、分子进化、蛋白质二级结构和结构域进行预测和推断。结果表明,3种作物二氢黄酮醇4-还原酶基因长度均约为1.0 kb,编码354个氨基酸,三者氨基酸同源性很高。在蛋白质的其他预测中,发现三者均为酸性稳定亲水性蛋白,没有信号肽和跨膜结构域。二级结构以α-螺旋和自由卷曲为主,在进化树中可以看出蓝粒小麦与普通小麦亲缘关系较近。通过分析发现,小麦类作物二氢黄酮醇4-还原酶有着相同的特性,进化比较保守。为进一步研究其他特殊的小麦类作物相关酶及其基因提供理论依据。To study the pigment synthesis＇ s related enzymes properties and genes of the color wheat, the dihydroflavonol-4-reductase genes of common wheat, blue-grained wheat, and quack grass were analyzed. The composition of amino acid sequences, molecular structure, physical and chemical characters, subcellular localization, transmembrane helices, and molecular phylogenetic evolution, secondary and tertiary structure from NCBI were analyzed and predicted by bioinformatics software. The results demonstrated that, the length of three DFR genes were all 1.0 kb, which coded 354 aa and had a high homology amino acid, and they were all acidic, hydrophilic, stable protein, had no signal peptide and across membrane structure. Secondary structures of the protein were most of alpha helix and random coil. In the evolutionary tree could be seen the blue-grained wheat and common wheat genetic relationship were close. The result implied the enzymes of DFR in the triticeae crops＇ properties was the same and had a conservative evolution. This study provided the basis of research the enzymes and their genes in further steo.

关 键 词：二氢黄酮醇4-还原酶 小麦 蓝粒小麦 麦类作物 生物信息学分析 

分 类 号：Q75[生物学—分子生物学]