显脉羊蹄甲的生物活性成分  

Bioactive constituents of Bauhinia glauca Benth. subsp. pernervosa

作　　者：汤迎湛[1] 邢亚超[1] 门瑞芝[1] 潘英[1] 孟大利[1] 李宁[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2014年第3期188-190,240,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(81001371)

摘　　要：目的对显脉羊蹄甲中具有抑制血小板聚集活性的有效部位的化学成分进行研究。方法从显脉羊蹄甲抑制血小板聚集活性部位中,利用溶剂分步萃取法和各种色谱手段(硅胶吸附柱色谱,聚酰胺柱色谱,Sephadex LH-20柱色谱,开放ODS柱色谱,液相色谱等)分离得到了10个化合物,根据其理化性质和光谱数据分析(1H-NMR、13C-NMR等)鉴定了它们的结构。结果从活性部位中分离鉴定的化合物为黄酮类,分别是:3,5,7,4'-四羟基-3'-甲氧基黄酮(3,5,7,4'-tetrahydroxy-3'-methoxyflavone,1)、5,7,3',4'-四羟基-3-甲氧基黄酮(5,7,3',4'-tetrahydroxy-3-methoxyflavone,2)、槲皮素(quercetin,3)、木樨草素(luteolin,4)、山柰酚(kaempferol,5)、5,7,4'-三羟基-3'-甲氧基黄酮(5,7,4'-trihydroxy-3'-methoxyflavone,6)、7,3',4'-三羟基-3-甲氧基黄酮(7,3',4'-trihydroxy-3-methoxyflavone,7)、杨梅苷(myricitrin,8)、槲皮素-3-O-α-L-吡喃阿拉伯糖苷(quercetin-3-O-α-Larabinopyranoside,9)、儿茶素(catechin,10)。结论化合物1-6、8-9均为首次从该植物中分离得到,化合物7为首次从羊蹄甲属植物中分离得到。Objective To isolate the bioactive constituents of Bauhinia glauca Benth. Subsp. pernervose T. Chen. Methods The active fraction were isolated by chromatography on silica gel and Sephadex LH-20 col- umn. The structure elucidation of ten compounds was carried out by analysis of the physico-chemical con- stants and the NMR data. Results The isolated compounds were identified as 3,5,7,4＇-tetrahydroxy-3＇-me- thoxyflavone （ 1 ）, 5,7,3＇, 4＇-tetrahydroxy-3-methoxyflavone （ 2 ）, quercetin （ 3 ）, luteolin （ 4 ）, kaempferol （ 5 ）, 5,7,4＇-trihydroxy -3＇-methoxyflavone （ 6 ）, 3,7,3＇-trihydroxy-4＇ -methoxyflavone （ 7 ）, myricitrin （ 8 ）, quercetin-3-O-α-L-arabinofuranoside （ 9 ） and （＋） catechin （ 10 ）. Conclusions Compounds 1- 6,8- 9 were i- solated from this plant firstly and compound 7 was reported from Bauhinia genus for the first time.

关 键 词：显脉羊蹄甲 生物活性成分 黄酮 

分 类 号：R284.1[医药卫生—中药学]