HPLC法同时测定益肝康颗粒中没食子酸、丹参素、芍药苷、丹酚酸B和丹参酮Ⅱ_A含量  

HPLC simultaneous determination of galic acid,tanshinol,paeoniflorin, salvianolic acid B and tanshinone Ⅱ_A in Yi gan kang granules

作　　者：崔晓红[1] 奇海涛[2] 生宁[2] 张兰桐[2] 田亚平[2] 郑旭光[2] 

机构地区：[1]河北医科大学第二医院药剂科,石家庄050017 [2]河北医科大学药学院药物分析教研室,石家庄050017

出　　处：《药物分析杂志》2014年第3期432-436,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：采用HPLC法同时测定益肝康颗粒（丹参、黄芪、赤芍、当归等）中活性成分没食子酸、丹参素、芍药苷、丹酚酸B和丹参酮Ⅱ。的含量。方法：采用c。。反相色谱柱，以甲醇-乙睛-0．1％磷酸水溶液为流动相梯度洗脱，检测波长为芍药苷230nm，没食子酸、丹参素、丹酚酸B、丹参酮IIA 270nm。结果：没食子酸、丹参素、芍药苷、丹酚酸B和丹参酮ⅡA进样量分别在30．1～376．8、452．7—5659．2、201．6～2520、1657．0～20712．0、54．336～679．2μg·mL^-1范围内呈良好的线性关系，平均回收率分别为102．5％、104．7％、99．4％、105．0％、102．9％。结论：该方法简捷，快速，可靠，可用于控制该制剂的质量。Objective： To develop a method for determining the contents of gallic acid,tanshinol, paeoniflorin, salvianolic acid B and tanshinone II A in Yi gan kang granules. Methods： The analytical column was an ODS col- umn. The mobile phase consisted of acetonitrile - 0. 1% phosphoric acid aqueous solution using gradient elution. The detection wavelength of paeoniflorin was 230 nm, and the wavelength for gallic acid, tanshinol, salvianolic acid B and tanshinone II A detection was set at 270 nm. Results ： The calibration curves of gallic acid, tanshinol, pae- oniflorin, salvianolic acid B and tanshinone II A showed good linearity in the ranges of 30. 1 - 376.8,452.7 - 5659.2,201.6 - 2520,1657.0 - 20712.0 and 54. 336 - 679.2 μg · mL-1 , and the average recoveries were 102. 5% , 104.7% ,99.4% , 105.0% and 102.9% , respectively. Conclusions ： The method is simple, rapid, reli- able and can be used for drug quality control. This method replenishes the insufficient quality control of Yi gan kang granules.

关 键 词：益肝康颗粒 没食子酸 丹参素 芍药苷 丹酚酸B 丹参酮ⅡA 含量测定 质量控制 HPLC—DAD 

分 类 号：R917[医药卫生—药物分析学]