促性腺激素释放激素拮抗剂对卵巢颗粒细胞分泌抗苗勒管激素的影响  

Effect of gonadotropin releasing hormone antagonist on the secretion of anti-Mullerian hormone of granulosa cells

作　　者：张英[1] 陆芝英[1] 王婷[1] 王佳佳[1] 华克勤[1] 

机构地区：[1]复旦大学附属妇产科医院,上海200011

出　　处：《中国妇幼保健》2014年第9期1417-1420,共4页Maternal and Child Health Care of China

基　　金：上海市卫生和计划生育委员会青年科研课题〔201344032〕

摘　　要：目的:研究促性腺激素释放激素拮抗剂(GnRH-ant)对卵巢颗粒细胞分泌抗苗勒管激素(AMH)的影响。方法:从人卵泡液中分离、培养颗粒细胞。分别用生理盐水(NS)、顺铂(cisplatin,DDP,5μg/ml)、cetrorelix(GnRH-ant,10-9mol/L)或cetrorelix(10-9mol/L)+DDP(5μg/ml)处理颗粒细胞24 h后,ELISA方法检测颗粒细胞分泌AMH的水平。裸鼠动物随机分为4组:NS组:皮下注射NS 0.1 ml/天;1周后腹腔注射NS 0.2 ml/周。DDP组:皮下注射NS 0.1 ml/天;1周后腹腔注射5 mg/kg DDP/周。cetrorelix组:皮下注射100μg cetrorelix/天;1周后腹腔注射NS 0.2 ml/周。cetrorelix+DDP组:皮下注射100μg cetrorelix/天;1周后腹腔注射5 mg/kg DDP/周。用药第29天,经眼球取血后用ELISA方法检测各组裸鼠血清AMH的水平。结果:成功分离、培养人卵巢颗粒细胞。与DDP组及NS组比较,cetrorelix组及cetrorelix+DDP组颗粒细胞AMH分泌明显增加(P<0.05)。Cetrorelix组及cetrorelix+DDP组裸鼠血清AMH水平明显高于DDP组(P<0.05)。结论:GnRHant能够上调颗粒细胞分泌AMH,对抗DDP对AMH的降调节作用。Objective： To research the effect of gonadotropin releasing hormone antagonist （ GnRH - ant） on the secretion of anti - Mullerian hormone （AMH） of granulosa cells. Methods：Human granulosa cells obtained from the follicular aspirates were cultured. The secretion of AMH in human granulosa cells administrated with GnRH - ant （ 10 - 9 mol/L） and/or platinum （5 μg/ml） compounds were detected by ELISA assay. A total of 24 nude mice were divided into4 groups： normal saline （NS） ： NS 0. 1 ml once a day subcutaneous injection and NS 0. 2 ml once a week peritoneal injection ; cisplatin （DDP） ： NS 0. 1 ml once a day subcutaneous injection and DDP 5 mg/kg （ diluted to 0. 2 ml） once a week peritoneal injection; cetrorelix： 100 μg cetrorelix （diluted to 0. 1 ml） once a day subcutaneous injection and NS 0. 2 ml once a week peritoneal injection; cetrorelix ＋ DDP：100 μg cetrorelix （diluted to 0. 1 ml） once a day subcutaneous injection and DDP 5 mg/kg （diluted to 0. 2 ml） once a week peritoneal injection; all the peritoneal injection started at one week after subcutaneous injection. Serum AMH levels were compared in the four groups. Results：Human granulosa cells were isolated and cultured successfully, AMH levels in cetrorelix group and cetrorelix ＋ DDP group obviously increased compared with NS group and DDP group （P 〈 0. 05 ）. The expres- sions of AMH in nude mice in cetrorelix group and cetrorelix ＋ DDP group were significantly higher than that in DDP group （P 〈 0. 05）. Conclusion：GnRH - ant can increase the secretion of AMH in granulosa ceils and inhibit the down - regulation effect of AMH induced by DDP.

关 键 词：促性腺激素释放激素拮抗剂 抗苗勒管激素 顺铂 卵巢 颗粒细胞 

分 类 号：R737.31[医药卫生—肿瘤]