原发性乳房外Paget病中HER-2的表达  

The expression of HER-2 in primary extramammary Paget's disease

作　　者：顾黎雄[1] 王美潭 曹双林[1] 吴晓琰[1] 

机构地区：[1]南通大学附属医院皮肤性病科,南通226001

出　　处：《南通大学学报（医学版）》2014年第1期5-7,F0002,共4页Journal of Nantong University(Medical sciences)

基　　金：南通市科技项目2010_K应用研究(K2010056)

摘　　要：目的:观察原发性乳房外Paget病中HER-2/neu基因扩增及HER-2蛋白的表达,探讨其在乳房外Paget病中的意义。方法:收集2007年1月—2011年9月原发性乳房外Paget病手术切除标本20份,应用显色原位杂交和免疫组织化学的方法,分别检测患者皮损组织石蜡包埋标本中HER-2/neu基因扩增和HER-2蛋白表达情况。结果:所有20例患者分为3组:免疫组织化学检测HER-2蛋白高表达组、低表达组和阴性表达组分别为8例、6例和6例,HER-2蛋白阳性表达率为70.0%。显色原位杂交检测高扩增组、低扩增组和无扩增组分别为7例、2例和11例,HER-2基因的扩增率为45.0%。采用Spearman秩相关进行分析,两种检测方法的结果具有正相关性(P<0.05)。结论:在原发性乳房外Paget病中存在HER-2/neu基因扩增和HER-2蛋白的表达,两者之间存在正相关性。干扰HER-2蛋白的表达可能为临床治疗原发性乳房外Paget病提供一种新的策略。Objective： To observe the HER-2 protein expression and HER-2/neu gene amplification in primary extramam-mary Paget’s disease（EMPD） and to investigate HER-2’s significance in Paget’s disease. Methods： A total of 20 cases of EMPD were enrolled into the study from Jan. 2007 to Sep. 2011. Immunohistochemistry（IHC） and chromogenic in situ hy-bridization（CISH） methods were used to detect the HER-2 protein expression and HER-2/neu gene amplification in paraffin-embedded surgical specimens respectively. Results：The results of HER-2 protein were divided into three groups：high expres-sion（8/20）, low expression（6/20） and negative expression（6/20） by IHC. The positive expression is up to 70%. The results of HER-2/neu gene were also divided into three groups： high amplification（7/20）, low amplification（2/20） and negative amplifi-cation（11/20） by CISH. The positive amplification reaches 45%. The correlation of HER-2 protein measured by IHC and HER-2/neu gene detected by CISH with Spearman rank correlation analysis , the result showed that the correlation of two methods is positive（P＆lt;0.05）. Conclusions： HER-2 protein expression and HER-2/neu gene amplification are existed in most cases of EMPD. The interference of HER-2 protein expression may provide a new strategy for the clinical management of EMPD.

关 键 词：PAGET病 乳房外 HER-2 NEU HER-2 显色原位杂交 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]