MiR-738通过Wnt信号通路调控斑马鱼心脏发育  

MiR-738 regulates the zebrafish heart formation through Wnt signaling

作　　者：施林生[1] 黄建飞[1] 卢辉和[1] 陈小丽[1] 郑扣龙[1] 林刚[1] 

机构地区：[1]江苏省南通市第一人民医院心内科,南通226001

出　　处：《南通大学学报（医学版）》2014年第1期8-10,F0002,共4页Journal of Nantong University(Medical sciences)

基　　金：南通市科技计划项目(BK2011057)

摘　　要：目的:研究miR-738对模式动物斑马鱼心脏发育的影响。方法:通过全胚胎原位杂交检测miR-738的表达。在斑马鱼受精卵中显微注射miR-738前体来过表达miR-738。通过显微镜成像来检测心脏表型。通过荧光素酶报告基因检测来验证miR-738调控Lef1。结果:MiR-738在30 hpf斑马鱼心脏表达。MiR-738过表达导致斑马鱼胚胎心脏显著增大。MiR-738可以抑制Lef1的表达。结论:MiR-738通过Wnt信号通路调控斑马鱼心脏发育。Objective： To examine the function of miR-738 involved in zebrafish heat development. Methods： By microinjec- tion of miR-738 precursor into zebrafish fertilized egg to overexpress miR-738. By microscope imaging to check the heart phe-notype. By using luciferase reporter assay to validate that miR-738 regulates Lefl directly. Results： MiR-738 was detected in zebrafish heart at 30 hpf. Overexpression of miR-738 resulted in the enlargement of zebrafish embryonic heart. MiR-738 in-hibits the Lefl expression. Conclusion： MiR-738 regulates the zebrafish heart formation through Wnt signaling.

关 键 词：miR-738 心脏 WNT 斑马鱼 发育 

分 类 号：R394.1[医药卫生—医学遗传学]