高脂培养抑制LPS诱导的HSC-T6细胞活化  

The Fat Culture Inhabited LPS-Induced Activation of HSCs-T6 Cells

作　　者：胡彬[1,2,3] 王赫[2,3] 晏贤春[2,3] 蒋芳[2,3] 王清明[2,3] 杨俊涛[2,3] 

机构地区：[1]中南大学生物科学与技术学院,湖南长沙410013 [2]蛋白质组学国家重点实验室,北京蛋白质组研究中心,国家蛋白质科学中心(北京),军事医学科学院放射与辐射医学研究所,北京102206 [3]蛋白质药物国家工程研究中心,北京102206

出　　处：《现代生物医学进展》2014年第8期1420-1422,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31000405);国家高技术研究发展计划(2012AA020201);重大科学仪器研究专项(2013YQ140405);国家"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2013ZX10002009-001)

摘　　要：目的:鉴定高脂培养对肝星形细胞活化的影响。方法:培养HSC-T6细胞系,加入含有游离脂肪酸的高脂培养基处理,利用LPS处理活化,通过检测α-SMA的表达分析星形细胞的活化程度,通过Q-PCR分析HSCs细胞胶原的表达,通过Q-PCR实验分析LPS相关通路靶基因表达情况情况。结果:高脂培养能够抑制LPS诱导的HSC-T6细胞增殖,降低HSC-T6细胞α-SMA和胶原I和TIMP-1表达的水平,Q-PCR的分析表明,高脂培养能够抑制HSCs活化后的NF-κB通路下游靶基因MCP-1和IL-6的表达。结论:在体外培养实验中,高脂培养能够抑制LPS诱导的HSC-T6细胞活化。Objective： To investigate the effect of high-fat medium on the activition of hepatic stellate cells. Methods： HSC-T6 cell line was cultured, and treated with high-fat medium which containing flee fatty acid, activated with LPS. The degree of activation was analyzed by detecting α-SMA expression. The expression of collagen-I and TIMP-1 was analyzed by Q-PCR, and the activation of transcription factor which related to LPS pathway was analyzed by Q-PCR assay. Results： High-fat medium was able to inhibit the activation of HSC-T6 cells which induced by LPS, reduced the expression of collagen level in the HSC-T6 cells. Q-PCR analysis showed that the high-fat medium inhibited the expression of MCP-1 and IL-6 which were the target genes of NF-KB in activated HSCs. Conclusion： In vitro culture experiments, high-fat medium can inhibit the activation of HSC-T6 cells which induced by LPS.

关 键 词：游离脂肪酸 肝星形细胞 LPS 肝纤维化 

分 类 号：Q593.1[生物学—生物化学] R575.2[医药卫生—消化系统]