辛热药药性表达与钙调蛋白之间关系的实验研究  

Experimental study on relationship between pungent-hot herb property express and calmodulin

作　　者：刘欣[1] 胡燕[1] 崔一然[2] 刘小青[2] 唐炳华[3] 张冰[2] 

机构地区：[1]兰州大学中西医结合研究所,甘肃兰州730000 [2]北京中医药大学中药学院,北京100102 [3]北京中医药大学基础医学院,北京100029

出　　处：《中国中药杂志》2014年第5期873-878,共6页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2007CB512605);高等学校博士学科点专项科研基金博导类课题(20100013110011);高等学校博士学科点专项科研基金新教师类课题(20120211120046);甘肃省科技计划项目(1208RJYA056);兰州大学自然科学类"中央高校基本科研业务费专项资金"自由探索项目-面上项目(lzujbky-2012-151;lzujbky-2012-153);北京中医药大学科研创新团队项目(2011-CXTD-14)

摘　　要：目的:在观察辛热药附子、仙茅、肉桂和苦寒药黄柏对阳虚状态大鼠下丘脑-垂体-靶腺轴功能调节的基础上,通过体内外研究其对钙调蛋白的作用,以期阐释辛热药药性表达的实质。方法:氢化可的松琥珀酸钠粉针剂肌肉注射复制阳虚大鼠模型,辛热药附子、仙茅、肉桂和苦寒药黄柏对其干预7 d后,检测下丘脑-垂体-靶腺轴的促甲状腺激素(TSH)、三碘甲腺原氨酸(T3)、甲状腺素4(T4)、17-羟皮质类固醇(17-OHCS)、皮质醇(COR)、睾酮(T)、雌二醇(E2)等相关指标及Western blot法检测各组肝脏和体外培养的L02细胞中钙调蛋白的表达。结果:辛热药附子、仙茅、肉桂对阳虚状态的下丘脑-垂体-靶腺轴指标、钙调蛋白均有明显的纠正作用,而苦寒药黄柏对其作用不明显。结论:初步确定辛热药药性表达与钙调蛋白密切相关。Objective： To explain the essence of pungent-hot herb property express according to in vivo and in vitro studies on its effect on calmodulin on the base of the observation of the adjustment in hypothalamic-pituitary-gonad axis functions of Aconiti Latera- lis Radix Praeparata, Curculiginis Rhizoma, Cinnamomi Cortex and bitter-cold herb Phellodendri Chinensis Cortex in rats under the state of yang deficiency. Method： The yang-deficient model was duplicated by intramuscularly injecting hydrocortisone sodium succi- Mate powder injection. After the intervention with Aconiti Lateralis Radix Praeparata, Curculiginis Rhizoma, Cinnamomi Cortex and bit- ter-cold herb Phellodendri Chinensis Cortex for seven days, TSH, T3 , T4, 17-OHCS, COR, T, E2 of hypothalamus-pituitary-target gland axis and other relevant indexes were detected. The calmodulin expression in livers and L02 cells cultured in vitro was detected by using Western blot. Result： Pungent-hot herbs Aconiti Lateralis Radix Praeparata, Curculiginis Rhizoma, Cinnamomi Cortex can significantly correct indicators of hypothalamic-pituitary-gonad axis and calmodulin, whereas the bitter-cold herb Phellodendri Chinensis Cortex showed no obvious effect. Conclusion： The pungent-hot herb property expression may be closely related to calmodulin.

关 键 词：辛热药 药性表达 钙调蛋白 阳虚状态 下丘脑-垂体-靶腺轴 

分 类 号：R285[医药卫生—中药学]